Farman N
Nephrologie. 1985;6(3):105-7.
The localization of mineralocorticoid and glucocorticoid binding sites along the nephron has been investigated by using an autoradiographic technique (dry film) and biochemical methods, applied on tubular segments of rabbit and rat kidney, isolated by microdissection, after in vitro incubation of kidney pyramids with tritiated aldosterone (Aldo) or dexamethasone (Dex). Based on analysis of dose-dependent specific nuclear binding values and competition studies, cortical collecting tubule (CCT) appears as the most specific mineralocorticoid segment; the distal tubule exhibits also high binding values, which are less specific for Aldo than in CCT; Aldo binding was lower in the ascending limb of Henle's loop and medullary collecting tubule and of mixed (gluco and mineralocorticoid) specificity. Aldo receptors were not present in proximal tubule and glomerulus. Specific nuclear binding of Dex was high all along the nephron, except in proximal tubules, and somewhat lower in glomeruli and its arterioles than in the distal parts of the nephron. Binding of 3H-Aldo did not differ drastically along the nephron of the rat and the rabbit.
利用放射自显影技术（干膜）和生化方法，对经微切割分离出的兔和大鼠肾的肾小管节段进行体外孵育，用氚标记的醛固酮（Aldo）或地塞米松（Dex）处理肾锥体后，研究了盐皮质激素和糖皮质激素结合位点在肾单位中的定位。基于剂量依赖性特异性核结合值分析和竞争研究，皮质集合管（CCT）似乎是最具特异性的盐皮质激素节段；远曲小管也表现出较高的结合值，但其对Aldo的特异性低于CCT；Henle袢升支和髓质集合管中Aldo结合较低，且具有混合（糖皮质激素和盐皮质激素）特异性。近端小管和肾小球中不存在Aldo受体。除近端小管外，Dex的特异性核结合在整个肾单位中都很高，在肾小球及其小动脉中的结合略低于肾单位远端部分。大鼠和兔肾单位中3H-Aldo的结合没有显著差异。
