Phillipson O T, Griffiths A C
Neuroscience. 1985 Oct;16(2):275-96. doi: 10.1016/0306-4522(85)90002-8.
Afferents to the nucleus accumbens have been studied with the retrograde transport of unconjugated wheatgerm agglutinin as detected by immunohistochemistry using the peroxidase-antiperoxidase method, in order to define precisely afferent topography from the cortex, thalamus, midbrain and amygdala. Cortical afferent topography was extremely precise. The largest number of cells was found following injections to the anterior accumbens. Anteromedial injections labelled a very large extent of the subiculum and part of the entorhinal cortex. Anterolateral injections produced less subicular and entorhinal label but also labelled the posterior perirhinal cortex. Posteromedial injections labelled only the ventral subiculum and a few cells in the adjacent medial entorhinal cortex. Posterolateral injections labelled few lateral entorhinal neurones but did label a long anteroposterior strip of perirhinal cortex. Prefrontal cortex label was found only after anterior accumbens injections. In the amygdala labelled neurones were found in cortical, central, lateral posterior, anteromedial and basolateral nuclei. Basolateral amygdala projected chiefly to the anteromedial accumbens and central nucleus to anterolateral accumbens. Only a weak amygdala label was found after posterior accumbens injections. In the ventral tegmental area, the midline interfascicular nucleus projected only to medial accumbens. The paranigral ventral tegmentum projected chiefly to the medial accumbens and the parabrachial area chiefly to the lateral accumbens. In the thalamus, heaviest label was found after anterior accumbens injections. Most cells were found in the paraventricular, reuniens and rhomboid nuclei and at posterior thalamic levels lying medial to the fasciculus retroflexus. There was only restricted topography found from thalamic sites. Retrograde label was also found in the ventral pallidum and lateral hypothalamus. Single small injection sites within accumbens received input from the whole anteroposterior extent of the thalamus and ventral tegmentum. The medial accumbens was found to have a close relationship to habenula, globus pallidus and interfascicular nucleus. It appeared that the heaviest volume of inputs projected to anteromedial accumbens, where output from hippocampus (CAI), subiculum, entorhinal and prefrontal cortices converged with output from amygdala, midline thalamus and ventral tegmentum.
为了精确确定来自皮质、丘脑、中脑和杏仁核的传入神经分布，运用过氧化物酶抗过氧化物酶法免疫组织化学检测，通过未结合的小麦胚凝集素逆行运输对伏隔核的传入神经进行了研究。皮质传入神经分布极其精确。在前部伏隔核注射后发现的细胞数量最多。前内侧注射标记了很大范围的下托和部分内嗅皮质。前外侧注射产生的下托和内嗅标记较少，但也标记了后梨状周皮质。后内侧注射仅标记了腹侧下托和相邻内侧内嗅皮质中的少数细胞。后外侧注射标记的外侧内嗅神经元较少，但确实标记了一条前后方向较长的梨状周皮质带。仅在前部伏隔核注射后才发现前额叶皮质有标记。在杏仁核中，在皮质核、中央核、外侧后核、前内侧核和基底外侧核中发现了标记神经元。基底外侧杏仁核主要投射到前内侧伏隔核，中央核投射到前外侧伏隔核。后部伏隔核注射后仅发现微弱的杏仁核标记。在腹侧被盖区，中线束间核仅投射到内侧伏隔核。黑质旁腹侧被盖主要投射到内侧伏隔核，臂旁区主要投射到外侧伏隔核。在丘脑中，前部伏隔核注射后发现的标记最重。大多数细胞位于室旁核、连合核和菱形核以及位于后屈束内侧的丘脑后部水平。从丘脑部位仅发现有限的分布情况。在腹侧苍白球和外侧下丘脑也发现了逆行标记。伏隔核内单个小注射部位接受来自丘脑和腹侧被盖前后全长的输入。发现内侧伏隔核与缰核、苍白球和束间核关系密切。似乎投射到前内侧伏隔核的输入量最大，海马（CA1）、下托、内嗅和前额叶皮质的输出与杏仁核、中线丘脑和腹侧被盖的输出在此汇聚。
