Kanagatov Z, Alpamyssova G, Nagiyeva A, Yeginbay A, Alpamyssova A, Abubakirova A
Zhetysu University named after I.Zhansugurov, Taldykorgan, Kazakhstan.
South Kazakhstan University of Muktar Auezov, Departament of Biotechnology, Shymkent, Kazakhstan.
Braz J Biol. 2025 Aug 8;85:e296200. doi: 10.1590/1519-6984.296200. eCollection 2025.
Cold stress significantly challenges agricultural productivity, particularly in regions susceptible to low temperatures. In response to this challenge, the current study investigates the effects of over-expressing the ICE1 gene in rice plants on cold tolerance. Through constructing the pCAMBIA3301-ICE1 vector, transgenic rice lines with increased ICE1 expression were generated and investigated for their response to chilling stress. Upon subjecting the transgenic rice plants to chilling stress conditions, notable improvements were observed compared to wild-type (WT) plants. Specifically, the transgenic lines exhibited a substantial 62% reduction in visual damage severity, highlighting their enhanced resilience to cold-induced damage. Additionally, the transgenic lines showed a significant 30% increase in proline concentrations, indicative of enhanced stress tolerance mechanisms. Further analyses revealed a 50% reduction in malondialdehyde (MDA) levels and a 38% increase in peroxidase (POX) activity in the transgenic rice plants compared to the WT plants. These findings suggest improved antioxidant defense mechanisms and reduced oxidative damage, contributing to the overall cold stress tolerance of the transgenic lines. Interestingly, differential expression patterns of the ICE1 gene were observed between the leaves and roots of the transgenic plants under cold stress. Roots consistently exhibited higher levels of ICE1 expression, highlighting potential organ-specific responses to cold stress. In conclusion, the study demonstrates that over-expression of the ICE1 gene significantly enhances cold stress tolerance in rice plants; offering promising avenues for developing stress-resistant crop varieties that can better withstand the adverse effects of chilling stress on agricultural production.
低温胁迫对农业生产力构成了重大挑战，尤其是在易受低温影响的地区。针对这一挑战，本研究调查了水稻植株中过表达ICE1基因对耐寒性的影响。通过构建pCAMBIA3301-ICE1载体，获得了ICE1表达增加的转基因水稻株系，并研究了它们对冷胁迫的反应。将转基因水稻植株置于冷胁迫条件下后，与野生型（WT）植株相比，观察到了显著的改善。具体而言，转基因株系的视觉损伤严重程度大幅降低了62%，突出了它们对冷害的更强恢复力。此外，转基因株系的脯氨酸浓度显著增加了30%，表明胁迫耐受机制得到增强。进一步分析发现，与WT植株相比，转基因水稻植株中的丙二醛（MDA）水平降低了50%，过氧化物酶（POX）活性增加了38%。这些发现表明抗氧化防御机制得到改善，氧化损伤减少，有助于转基因株系的整体耐寒性。有趣的是，在冷胁迫下，转基因植株的叶片和根之间观察到ICE1基因的差异表达模式。根始终表现出较高水平的ICE1表达，突出了对冷胁迫潜在的器官特异性反应。总之，该研究表明ICE1基因的过表达显著增强了水稻植株的耐寒性；为培育能够更好地抵御冷胁迫对农业生产不利影响的抗逆作物品种提供了有前景的途径。
