生物体液中人类血红蛋白的免疫化学测定（作者译）
[Immunochemical determination of human hemoglobin in biological fluids (author's transl)].
作者信息
Engler R, Pointis J, Rondeau Y, Jundon C, Waks M
出版信息
Clin Chim Acta. 1977 Jun 1;77(2):159-65. doi: 10.1016/0009-8981(77)90023-7.
PMID:408050
Abstract
An assay of human hemoglobin by immunonephelometry-electroimmunodiffusion is proposed. Concentrations in the range from 5 to 180 mg% can be determined to within 2--5% accuracy. The immunonephelometric assay is independent of the presence of haptoglobin whereas the electroimmunodiffusion assay is not. This immunochemical assay, independent of the peroxydase activity of hemoglobin, can be carried out in any biological fluid (plasma, urine, gastric fluid).
摘要
提出了一种通过免疫比浊法-电免疫扩散法测定人血红蛋白的方法。浓度在5至180mg%范围内时，测定精度可达2%-5%。免疫比浊法测定不受触珠蛋白存在的影响，而电免疫扩散法受其影响。这种免疫化学测定法不受血红蛋白过氧化物酶活性的影响，可在任何生物体液（血浆、尿液、胃液）中进行。
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