Yang Wenjuan, Guo Ming, Li Yan, Yang Qinglan, Zhang Huaizhi, Li Chengdao, Wang Juncheng, Meng Yaxiong, Ma Xiaole, Li Baochun, Yao Lirong, Zhang Hong, Yang Ke, Shang Xunwu, Si Erjing, Wang Huajun
State Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou 730070, China.
College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
Plants (Basel). 2025 Jul 30;14(15):2350. doi: 10.3390/plants14152350.
Barley leaf stripe, caused by (), significantly reduces yields across various regions globally. Understanding the resistance mechanisms of barley to is crucial for advancing disease resistance breeding efforts. In this study, two barley genotypes-highly susceptible Alexis and immune Ganpi2-were inoculated with the highly pathogenic isolate QWC for 7, 14, and 18 days. The number of differentially expressed genes (DEGs) in Alexis was 1350, 1898, and 2055 at 7, 14, and 18 days, respectively, while Ganpi2 exhibited 1195, 1682, and 2225 DEGs at the same time points. Gene expression pattern analysis revealed that Alexis responded more slowly to infection compared to Ganpi2. A comparative analysis identified 457 DEGs associated with Ganpi2's immunity to . Functional enrichment of these DEGs highlighted the involvement of genes related to plant-pathogen interactions and kinase activity in immunity. Additionally, 20 resistance genes and 24 transcription factor genes were predicted from the 457 DEGs. Twelve candidate genes were selected for qRT-PCR verification, and the results showed that the transcriptomic data was reliable. We conducted cloning of the candidate resistance gene by the barley cultivar Ganpi2, and the sequence analysis confirmed that the gene contains seven leucine-rich repeat (LRR) domains and an S_TKc domain. Subcellular localization in tobacco indicates that the HvLRR_8-1 is localized on the cell membrane. Through the functional analysis using virus-induced gene silencing, it was demonstrated that plays a critical role in regulating barley resistance to . This study represents the first comparative transcriptome analysis of barley varieties with differing responses to infection, providing that represents a promising candidate gene for improving durable resistance against in cultivated barley.
大麦叶条纹病由（）引起，在全球不同地区均显著降低产量。了解大麦对（该病害）的抗性机制对于推进抗病育种工作至关重要。在本研究中，用高致病性（病原菌）分离株QWC分别接种两个大麦基因型——高感品种Alexis和免疫品种甘啤2号，接种时间为7天、14天和18天。Alexis在接种7天、14天和18天时，差异表达基因（DEG）的数量分别为1350个、1898个和2055个，而甘啤2号在相同时间点的DEG数量分别为1195个、1682个和2225个。基因表达模式分析表明，与甘啤2号相比，Alexis对（病原菌）感染的反应较慢。比较分析确定了457个与甘啤2号对（该病害）免疫相关的DEG。这些DEG的功能富集突出了与植物-病原体相互作用和激酶活性相关的基因在（该病害）免疫中的作用。此外，从457个DEG中预测出20个抗性基因和24个转录因子基因。选择12个候选基因进行qRT-PCR验证，结果表明转录组数据可靠。我们通过大麦品种甘啤2号对候选（抗病）基因进行克隆，序列分析证实该（抗病）基因包含7个富含亮氨酸重复序列（LRR）结构域和一个S_TKc结构域。在烟草中的亚细胞定位表明HvLRR_8-1定位于细胞膜上。通过病毒诱导基因沉默进行功能分析表明，（该基因）在调节大麦对（该病害）的抗性中起关键作用。本研究是对感染（该病害）反应不同的大麦品种的首次比较转录组分析，表明（该基因）是提高栽培大麦对（该病害）持久抗性的一个有前景的候选基因。
