Gansu Provincial Key Laboratory of Aridland and Crop Science, Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou 730070, China; College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
Gansu Provincial Key Laboratory of Aridland and Crop Science, Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou 730070, China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
Plant Sci. 2025 Jan;350:112308. doi: 10.1016/j.plantsci.2024.112308. Epub 2024 Oct 28.
Barley leaf stripe is an important disease caused by Pyenophora graminea that affects barley yields in the world. Ascorbic acid (AsA) interacts with key elements of a complex network orchestrating plant defense mechanisms, thereby influencing the outcome of plant-pathogen interaction. Myo-inositol oxygenase (MIOX) is a pivotal enzyme involved in plants development and environmental stimuli. However, MIOX has described functions in plants but has not been characterized in fungi. In this study, we characterized the Pgmiox gene in P. graminea pathogenesis through annotated on the metabolic pathway of ascorbic acid aldehyde. Our analysis suggested that the Pgmiox protein had a typical conserved MIOX domain. Multiple alignment analysis indicated that the P. graminea MIOX orthologue clustered with MIOX proteins of Pyrenophora species. RNA interference successfully reduced transcript abundance of Pgmiox in six transformant lines compared to wild type, and the transformants were further less virulent on the host plant barley. Transformants of Pgmiox had significant reductions in vegetative growth and pathogenicity, which had increased resistance to tebuconazole and carbendazim. In addition, Pgmiox is associated with ionic, drought, osmotic, oxidative, and heavy metal stress tolerance in P. graminea. In conclusion, our findings reveal that Pgmiox may be widely utilized by fungi to enhance pathogenesis and holds significant potential for the development of durable P. graminea resistance through genetic modifications.
大麦条纹病是由禾谷布氏白粉菌引起的一种重要病害，影响世界范围内大麦的产量。抗坏血酸（AsA）与调控植物防御机制的复杂网络中的关键元素相互作用，从而影响植物-病原体相互作用的结果。肌醇氧合酶（MIOX）是参与植物发育和环境刺激的关键酶。然而，MIOX 在植物中有描述的功能，但在真菌中尚未被描述。在这项研究中，我们通过对抗坏血酸醛代谢途径的注释，对禾谷布氏白粉菌发病机制中的 Pgmiox 基因进行了表征。我们的分析表明，Pgmiox 蛋白具有典型的保守 MIOX 结构域。多重比对分析表明，禾谷布氏白粉菌的 MIOX 同源物与 Pyrenophora 属的 MIOX 蛋白聚类。与野生型相比，RNA 干扰成功地降低了六个转化株系中 Pgmiox 的转录丰度，并且转化株系在大麦宿主植物上的毒力进一步降低。Pgmiox 的转化体在营养生长和致病性方面有显著降低，对戊唑醇和多菌灵的抗性增加。此外，Pgmiox 与禾谷布氏白粉菌的离子、干旱、渗透、氧化和重金属胁迫耐受有关。总之，我们的研究结果表明，Pgmiox 可能被真菌广泛用于增强致病性，并为通过遗传修饰提高禾谷布氏白粉菌的持久抗性提供了重要潜力。
