Joshi Soniya, Huo Chen, Budhathoki Rabin, Gurung Anita, Bhattarai Salyan, Sharma Khaga Raj, Kim Ki Hyun, Parajuli Niranjan
Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu 44618, Nepal.
School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea.
Plants (Basel). 2025 Aug 2;14(15):2395. doi: 10.3390/plants14152395.
A comprehensive metabolic profiling of (L.) G. Don was performed using tandem mass spectrometry, along with an evaluation of the biological activities of its various solvent extracts. Among these, the methanolic leaf extract exhibited mild radical scavenging activity, low to moderate antimicrobial activity, and limited cytotoxicity in both the brine shrimp lethality assay and MTT assay against HeLa and A549 cell lines. High-performance liquid chromatography-electrospray ionization-high-resolution tandem mass spectrometry (HPLC-ESI-HRMS/MS) analysis led to the annotation of 34 metabolites, primarily alkaloids. These included 23 indole alkaloids, two fatty acids, two pentacyclic triterpenoids, one amino acid, four porphyrin derivatives, one glyceride, and one chlorin derivative. Notably, two metabolites-2,3-dihydroxypropyl 9,12,15-octadecatrienoate and (10)-hydroxypheophorbide A-were identified for the first time in . Furthermore, Global Natural Products Social Molecular Networking (GNPS) analysis revealed 18 additional metabolites, including epoxypheophorbide A, 11,12-dehydroursolic acid lactone, and 20-isocatharanthine. These findings highlight the diverse secondary metabolite profile of and support its potential as a source of bioactive compounds for therapeutic development.
使用串联质谱对（L.）G. Don进行了全面的代谢谱分析，并对其各种溶剂提取物的生物活性进行了评估。其中，甲醇叶提取物在卤虫致死试验以及针对HeLa和A549细胞系的MTT试验中表现出轻度的自由基清除活性、低至中度的抗菌活性和有限的细胞毒性。高效液相色谱-电喷雾电离-高分辨率串联质谱（HPLC-ESI-HRMS/MS）分析鉴定出34种代谢物，主要为生物碱。这些包括23种吲哚生物碱、两种脂肪酸、两种五环三萜、一种氨基酸、四种卟啉衍生物、一种甘油酯和一种二氢卟吩衍生物。值得注意的是，两种代谢物——2,3-二羟基丙基9,12,15-十八碳三烯酸酯和（10）-羟基脱镁叶绿酸A——首次在（L.）G. Don中被鉴定出来。此外，全球天然产物社会分子网络（GNPS）分析还揭示了另外18种代谢物，包括环氧脱镁叶绿酸A、11,12-脱氢乌索酸内酯和20-异长春花碱。这些发现突出了（L.）G. Don多样的次生代谢物谱，并支持其作为治疗开发中生物活性化合物来源的潜力。
