Martinez-Gutierrez Alfredo, Cami Helena, Noya Teresa, Gómez-Escalante Susana, Miró Llosas Aina, González Mari Carmen
Biotechnology Unit, Mesoestetic Pharma Group, 08840 Barcelona, Spain.
Medical Affairs, Mesoestetic Pharma Group, 08840 Barcelona, Spain.
Int J Mol Sci. 2025 Aug 5;26(15):7548. doi: 10.3390/ijms26157548.
Various biomaterials are currently employed for dermal biostimulation and filling purposes, with hyaluronic acid (HA)-based fillers among those with the most favorable safety profile, albeit exhibiting a limited biostimulatory effect. This study suggests that hyaluronic acid and succinic acid (SA) can significantly induce beneficial effects on skin cells by targeting key aging hallmarks. Human dermal senescent fibroblasts and aged adipocytes were treated with HA + SA, and various aging characteristics were examined through gene expression analysis and microscopy staining. HA was found to stimulate autophagy gene expression, while SA modulated senescence-gene expression, and the combination of these compounds induced mitophagy in senescent fibroblasts. Additionally, the HA + SA promoted adipogenesis, increased , and decreased gene expression in aged adipocytes. Furthermore, the conditioned medium from adipocytes treated with HA + SA upregulated key dermal genes such as and . The findings of this study suggest that HA and SA compounds can be used for the biostimulation of aged skin through the regulation of senescence-associated gene expression and cell communication between dermal fibroblasts and subcutaneous adipocytes.
目前，各种生物材料被用于皮肤生物刺激和填充目的，基于透明质酸（HA）的填充剂具有最良好的安全性，尽管其生物刺激作用有限。本研究表明，透明质酸和琥珀酸（SA）可通过针对关键衰老特征对皮肤细胞产生显著有益影响。用HA + SA处理人皮肤衰老成纤维细胞和老化脂肪细胞，并通过基因表达分析和显微镜染色检查各种衰老特征。发现HA刺激自噬基因表达，而SA调节衰老基因表达，这些化合物的组合诱导衰老成纤维细胞中的线粒体自噬。此外，HA + SA促进老化脂肪细胞的脂肪生成，增加并降低基因表达。此外，用HA + SA处理的脂肪细胞的条件培养基上调了关键的皮肤基因，如和。本研究结果表明，HA和SA化合物可通过调节衰老相关基因表达以及皮肤成纤维细胞与皮下脂肪细胞之间的细胞通讯，用于老年皮肤的生物刺激。
