Shuvo Abdullah Al Sufian, Kassai Masahiro, Kawahara Takeshi
Division of Biological and Agricultural Sciences, Department of Science and Technology, Graduate School of Medicine, Science and Technology, Shinshu University, Minami-minowa, Kami-ina, Nagano 399-4598, Japan.
S&B Foods Inc., #605 Mitsui Link-Lab Shinkiba1 Shinkiba 2-3-8, Koto-ku, Tokyo 136-0082, Japan.
Foods. 2025 Aug 6;14(15):2744. doi: 10.3390/foods14152744.
The induction of type I interferon (IFN) via intracellular nucleic acid sensors may be useful in preventing viral infections. However, little is known about the effect of natural plant materials on sensor responses. We previously found that cardamom ( (L.) Maton) seed extract (CSWE) enhanced type I IFN expression and prevented influenza virus infection. In this study, we investigated the effect of CSWE on type I IFN responses using intracellular nucleic acid sensor molecules. Human lung epithelial A549 cells were treated with CSWE and transfected with poly(dA:dT) or poly(I:C) using lipofection. CSWE and 1,8-cineole, the major CSWE components, dose-dependently induced type I IFNs and IFN-stimulated genes in both poly(dA:dT)- and poly(I:C)-transfected A549 cells. The type I IFN-enhancing effect of CSWE was dependent on the stimulator of interferon genes (STING), whereas the effect of 1,8-cineole was independent of STING and mediated by the down-regulation of 2,3,7,8-tetrachlorodibenzo--dioxin (TCDD)-inducible poly-ADP-ribose polymerase expression. Our study suggests that CSWE has the potential to act as a beneficial antiviral agent by enhancing homeostatic type I IFN production.
通过细胞内核酸传感器诱导I型干扰素（IFN）可能有助于预防病毒感染。然而，关于天然植物材料对传感器反应的影响知之甚少。我们之前发现小豆蔻（(L.) Maton）种子提取物（CSWE）可增强I型干扰素表达并预防流感病毒感染。在本研究中，我们使用细胞内核酸传感器分子研究了CSWE对I型干扰素反应的影响。用CSWE处理人肺上皮A549细胞，并使用脂质转染法用聚（dA:dT）或聚（I:C）进行转染。CSWE及其主要成分1,8-桉叶素在聚（dA:dT）和聚（I:C）转染的A549细胞中均呈剂量依赖性地诱导I型干扰素和干扰素刺激基因。CSWE增强I型干扰素的作用依赖于干扰素基因刺激物（STING），而1,8-桉叶素的作用不依赖于STING，是通过下调2,3,7,8-四氯二苯并-对-二恶英（TCDD）诱导的聚ADP-核糖聚合酶表达介导的。我们的研究表明，CSWE有潜力通过增强内稳态I型干扰素的产生而作为一种有益的抗病毒剂。
