Rolandelli R H, Saul S H, Settle R G, Jacobs D O, Singer R L, Wolf G L, Rombeau J L
Physiol Chem Phys Med NMR. 1985;17(2):197-203.
The present study determined whether in vitro nuclear magnetic resonance could be used to assess experimentally induced colitis in rats. Acute colitis was induced in 6 Sprague-Dawley rats by acetic acid enema, while 6 control animals received saline enemas. All animals were sacrificed 24 hours post-enema, and NMR relaxation times, T1 and T2, of colonic samples were determined on a 10 MHz spin analyzer (RADX, Houston, TX). Colonic water content was determined on the same samples by desiccation. Colitis animals showed significantly higher T1 and T2 relaxation times and tissue water content than controls. T1 and T2 times correlated significantly with tissue water content. Twelve additional animals were studied histologically, six of which received acetic acid enemas and showed extensive transmural colitis. Our results suggest that in vivo proton NMR might be a useful means of non-invasively assessing the degree of colonic inflammation.
本研究确定了体外核磁共振是否可用于评估实验诱导的大鼠结肠炎。通过乙酸灌肠在6只Sprague-Dawley大鼠中诱导急性结肠炎，而6只对照动物接受生理盐水灌肠。灌肠后24小时处死所有动物，并在10 MHz自旋分析仪（RADX，休斯顿，德克萨斯州）上测定结肠样本的NMR弛豫时间T1和T2。通过干燥测定相同样本的结肠含水量。结肠炎动物的T1和T2弛豫时间以及组织含水量显著高于对照组。T1和T2时间与组织含水量显著相关。另外对12只动物进行了组织学研究，其中6只接受乙酸灌肠并表现出广泛的透壁性结肠炎。我们的结果表明，体内质子核磁共振可能是一种非侵入性评估结肠炎症程度的有用方法。
