Kaensa Wiwat, Charoensuk Lakhanawan, Nakmai Chadon, Pinlaor Somchai, Suwannatrai Apiporn T, Laothong Umawadee, Prakobwong Suksanti
Department of Biology, The Eco-Health and Parasitology Research Unit, Faculty of Science, Udon Thani Rajabhat University, Udon Thani 41000, Thailand.
Department of Clinical Pathology, Faculty of Medicine Vajira Hospital, Navamindradhiraj University, Bangkok 10300, Thailand.
Parasite Epidemiol Control. 2025 Jul 2;30:e00447. doi: 10.1016/j.parepi.2025.e00447. eCollection 2025 Aug.
Opisthorchiasis, caused by , remains a public health concern in Southeast Asia, particularly in Thailand. This study investigates the prevalence and intensity of metacercariae in cyprinoid fish from the Nong Han Wetland in Northeast Thailand, and assesses fish abundance and environmental factors influencing parasite distribution. Fish were sampled monthly at eight sites during the rainy (June - October 2024) and dry (November 2024 - March 2025) seasons. Fish species were identified, and their relative abundance and species diversity (Shannon-Wiener index (H')) were assessed. Cyprinoid fish were digested with 0.25 % pepsin-HCl to detect, identified and quantify metacercariae under a microscope. Concurrent water quality paramteters (temperature, pH, dissolved oxygen, and fecal coliform levels) were analyzed to assess their correlation with parasite distribution. A total of 1429 fish from 36 species were collected, with (Family Belontiidae) being the most abundant. Among 392 cyprinoid fish (Family Cyprinidae) from 11 species (H' = 1.8), 15.1 % were infected with metacercariae found in 6 species. showed the significant highest prevalence (20.3 %). Infection intensity was highest in and (6.0 metacercariae/infected fish). Higher fecal coliform levels and proximity to human settlements correlated with infection. Dry season prevalence (17.8 %) and intensity (5.1 ± 2.6) were significantly greater than the rainy season (11.0 % and 2.6 ± 1.1, ≤ 0.001). A strong correlation existed between fecal coliforms and prevalence ( = 0.981, ≤ 0.001), with hotspots near human settlements. The findings highlight the influence of fecal contamination, seasonality, and human factors on transmission, emphasizing the need for targeted interventions.
由[未提及具体病原体名称]引起的后睾吸虫病仍是东南亚地区，尤其是泰国的一个公共卫生问题。本研究调查了泰国东北部农汉湿地鲤科鱼类中后睾吸虫囊蚴的流行率和感染强度，并评估了鱼类丰度以及影响寄生虫分布的环境因素。在雨季（2024年6月至10月）和旱季（2024年11月至2025年3月）期间，每月在八个地点对鱼类进行采样。鉴定鱼类物种，并评估其相对丰度和物种多样性（香农 - 维纳指数（H'））。用0.25%的胃蛋白酶 - 盐酸消化鲤科鱼类，以便在显微镜下检测、鉴定和定量后睾吸虫囊蚴。同时分析水质参数（温度、pH值、溶解氧和粪大肠菌群水平），以评估它们与寄生虫分布的相关性。共收集了来自36个物种的1429条鱼，其中[未提及具体物种名称，可能是应为“斗鱼科（Belontiidae）”]最为丰富。在来自11个物种（H' = 1.8）的392条鲤科鱼类（鲤科）中，15.1%感染了后睾吸虫囊蚴，发现于6个物种中。[未提及具体物种名称]的流行率显著最高（20.3%）。感染强度在[未提及具体物种名称]和[未提及具体物种名称]中最高（6.0个后睾吸虫囊蚴/感染鱼）。较高的粪大肠菌群水平和靠近人类住区与感染相关。旱季的流行率（17.8%）和感染强度（5.1±2.6）显著高于雨季（11.0%和2.6±1.1，P≤0.001）。粪大肠菌群与后睾吸虫流行率之间存在很强的相关性（r = 0.981，P≤0.001），人类住区附近存在热点。研究结果突出了粪便污染、季节性和人为因素对后睾吸虫传播的影响，强调了有针对性干预措施的必要性。
