中国浙江省养殖牛、山羊和猪中[具体对象未明确,可能是某种病原体之类]的分子鉴定与基因分型
Molecular identification and genotyping of in farmed Cattle, Goats, and Pigs from Zhejiang Province, China.
作者信息
Zhao Wei, Sun Lijie, Sun Yanbin, Fu Xinyi, Ma Shiyang, Zhang Jiayin, Yan Baolong
机构信息
School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, China.
Department of Clinical Laboratory, The Fifth Affiliated Hospital Sun Yat-sen University, Zhuhai 519000, China.
出版信息
Food Waterborne Parasitol. 2025 Aug 6;40:e00280. doi: 10.1016/j.fawpar.2025.e00280. eCollection 2025 Sep.
is a genus of parasitic protozoa that parasitize/colonize humans and animals gastrointestinal tract. The current study performed a molecular survey of in farm cattle (), goats (), and pigs () raised in different cities in Zhejiang Province of China to better understand the epidemiology of in the animals of this region. A total of 859 fresh fecal samples were collected from 265 cattle, 386 goats, and 208 pigs. All these samples were screened for the detection of by amplifying the small subunit ribosomal RNA () gene PCR and Sanger sequencing. Of the 859 samples tested, 12.1 % (104) were positive for , with cattle showing a prevalence of 19.6 % (52/265), goats with 11.4 % (44/386), and pigs with 3.8 % (8/208). Eight different subtypes of were found: ST10 ( = 51), ST5 ( = 11), ST12 ( = 9), ST14 ( = 8), ST21 ( = 8), ST23 ( = 7), ST26 ( = 6), and ST4 ( = 4). Cattle carried seven (ST5, ST10, ST12, ST14, ST21, ST23, and ST26), whereas goats harbored eight (ST4, ST5, ST10, ST12, ST14, ST21, ST23, and ST26) subtypes. All pig-derived isolates belonged only to ST5. These results are significant as they indicate that cattle, goats, and pigs in Zhejiang Province, China, harbor various subtypes of , which enhances our understanding of the distribution of among these hosts in China.
是一种寄生原生动物属,寄生于/定殖于人和动物的胃肠道。当前研究对中国浙江省不同城市饲养的肉牛()、山羊()和猪()进行了分子调查,以更好地了解该地区动物中的流行病学情况。共从265头牛、386只山羊和208头猪中采集了859份新鲜粪便样本。通过聚合酶链反应(PCR)扩增小亚基核糖体RNA()基因并进行桑格测序,对所有这些样本进行检测以筛查。在检测的859份样本中,12.1%(104份)呈阳性,其中牛的感染率为19.6%(52/265),山羊为11.4%(44/386),猪为3.8%(8/208)。发现了8种不同的亚型:ST10(=51)、ST5(=11)、ST12(=9)、ST14(=8)、ST21(=8)、ST23(=7)、ST26(=6)和ST4(=4)。牛携带7种(ST5、ST10、ST12、ST14、ST21、ST23和ST26),而山羊携带8种(ST4、ST5、ST10、ST12、ST14、ST21、ST23和ST26)亚型。所有猪源分离株仅属于ST5。这些结果意义重大,因为它们表明中国浙江省的牛、山羊和猪携带多种亚型,这增进了我们对在中国这些宿主中分布情况的了解。
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