Duan Junxia, Qin Huikai, Sun Mengqing, Fu Yin, Lang Jiashu, Zhang Aihui, Qin Ziyang, Guo Zhenxuan, Xu Huiyan, Li Xiaoying, Wang Chunren, Zhang Longxian
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, PR China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450046, Henan Province, China; Key Laboratory of Quality and Safety Control of Poultry Products (Zhengzhou), Ministry of Agriculture and Rural Affairs, PR China.
Key Laboratory of Bovine Disease Control in Northeast China, Ministry of Agriculture and Rural affairs, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, PR China.
Parasitol Int. 2024 Jun;100:102871. doi: 10.1016/j.parint.2024.102871. Epub 2024 Feb 24.
Blastocystis sp., Enterocytozoon bieneusi, and Giardia duodenalis are three common zoonotic intestinal parasites, and cattle are important hosts of these three intestinal protozoa. In this study, 1632 fecal samples were collected from dairy farms in Heilongjiang Province, China, and screened for Blastocystis sp., E. bieneusi, and G. duodenalis using polymerase chain reaction. Of these, 149 (9.13%) were positive for three zoonotic pathogens, including 104 (6.40%), 22 (1.35%), and 23 (1.41%) for Blastocystis sp., E. bieneusi, and G. duodenalis, respectively. Based on partial SSU rRNA gene sequencing analysis, 104 positive samples of Blastocystis sp. were found, and a total of nine known subtypes were identified, including ST10 (61), ST3 (18), ST14 (6), ST26 (7), ST24 (3), ST25 (2), ST1 (2), ST5 (2), and ST21 (1). Among these, three subtypes (ST1, ST3, and ST5) were recognized as zoonotic subtypes, and two subtypes (ST10 and ST14) were specific to animals. All 23 Giardia duodenalis-positive samples belonged to assemblage E (n = 23) based on sequenced beta-giardin (bg) and triosephosphate isomerase (tpi) genes. Three known genotypes of E. bieneusi, namely J (n = 9), I (n = 6), and BEB4 (n = 7), were identified by sequence analysis of the internal transcriptional spacer region gene. Our study provides basic data for prevention and control in Heilongjiang Province; however, further research is required to better understand the prevalence and public health significance of these pathogens in the Heilongjiang region.
芽囊原虫属、微小隐孢子虫和十二指肠贾第虫是三种常见的人畜共患肠道寄生虫，牛是这三种肠道原生动物的重要宿主。在本研究中，从中国黑龙江省的奶牛场收集了1632份粪便样本，并使用聚合酶链反应对芽囊原虫属、微小隐孢子虫和十二指肠贾第虫进行筛查。其中，149份（9.13%）对三种人畜共患病原体呈阳性，包括芽囊原虫属104份（6.40%）、微小隐孢子虫22份（1.35%）和十二指肠贾第虫23份（1.41%）。基于部分小亚基核糖体RNA（SSU rRNA）基因测序分析，发现了104份芽囊原虫属阳性样本，共鉴定出9种已知亚型，包括ST10（61份）、ST3（18份）、ST14（6份）、ST26（7份）、ST24（3份）、ST25（两份）、ST1（两份）、ST5（两份）和ST（21份）。其中，三种亚型（ST1、ST3和ST5）被认为是人畜共患亚型，两种亚型（ST10和ST14）是动物特有的。根据测序的β-贾第蛋白（bg）和磷酸丙糖异构酶（tpi）基因，所有23份十二指肠贾第虫阳性样本均属于E群（n = 23）。通过内部转录间隔区基因序列分析，鉴定出微小隐孢子虫的三种已知基因型，即J型（n = 9）、I型（n = 6）和BEB型（n = 7）。我们的研究为黑龙江省的防控提供了基础数据；然而，需要进一步研究以更好地了解这些病原体在黑龙江地区的流行情况和公共卫生意义。
