Arundel C M, Glicksman A S, Leith J T
Radiat Res. 1985 Dec;104(3):443-8.
Two subpopulations of human colon tumor cells (clones A and D) which differ in their intrinsic sensitivity to X irradiation were grown for several passages in tissue culture medium containing the differentiation-inducing agent sodium butyrate (NaB, 2 mM). Values of the single-hit, multitarget survival curve parameters for non-NaB-treated clone A cells were n = 17.1, D0(Gy) = 0.81, and DQ(Gy) = 2.31; corresponding parameters for NaB-treated cells were 1.04, 1.16, and 0.05. For non-NaB-treated clone D cells, the survival parameters were n = 4.27, D0 = 1.05, and DQ = 1.52; corresponding parameters for NaB-treated cells were 1.19, 1.15, and 0.20. The large reduction in the DQ parameters of both clone A and D cells after NaB treatment indicates that sodium butyrate-induced cell maturation is accompanied by increase in radiation cell kill, particularly in the low-dose region of the survival curve.
两种对X射线内在敏感性不同的人结肠肿瘤细胞亚群（克隆A和克隆D）在含有分化诱导剂丁酸钠（NaB，2 mM）的组织培养基中传代培养了几代。未用NaB处理的克隆A细胞的单击多靶存活曲线参数值为n = 17.1，D0（Gy） = 0.81，DQ（Gy） = 2.31；用NaB处理的细胞的相应参数为1.04、1.16和0.05。对于未用NaB处理的克隆D细胞，存活参数为n = 4.27，D0 = 1.05，DQ = 1.52；用NaB处理的细胞的相应参数为1.19、1.15和0.20。NaB处理后克隆A和克隆D细胞的DQ参数大幅降低，表明丁酸钠诱导的细胞成熟伴随着辐射细胞杀伤的增加，特别是在存活曲线的低剂量区域。
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