Photosynthesis by isolated chloroplasts of Sorghum vulgare.

The ambiguous location of photosynthetic carboxylases of mesophyll and bundle sheath chloroplasts of sorghum was investigated after successful homogeneous preparation. The phosphoenol pyruvate carboxylase was found as a particulate enzyme in the mesophyll cell chloroplasts and ribulose 1,5-biphosphate carboxylase in the stroma of the bundle sheath cell chloroplasts. Extensive characterization was carried out on these 2 enzymes for better understanding of the enzyme action.