Abraham K A, Pihl A
Eur J Biochem. 1977 Aug 1;77(3):589-93. doi: 10.1111/j.1432-1033.1977.tb11703.x.
An enzymic procedure was used to remove the 7-methylguanosine diphosphate moiety at the 5' ends of rabbit hemoglobin mRNA and mouse immunoglobulin light-chain mRNA. Evidence was obtained that the procedure, which involves the use of polynucleotide kinase, does not result in any further degradation of the mRNA. The enzymically decapped mRNA was as effective as untreated mRNA in supporting protein synthesis in a wheat germ system. This was the case over a wide range of mRNA concentrations and over a considerable period of time. The presence in the incubation mixture of S-adenosylhomocysteine, an inhibitor of methylation, did not affect the results. The data indicate that the presence of a 7-methylguanosine diphosphate residue at the 5' end of mRNAs is not an obligatory requirement for translation in eucaryotic systems.
采用酶促方法去除兔血红蛋白mRNA和小鼠免疫球蛋白轻链mRNA 5'端的7-甲基鸟苷二磷酸部分。有证据表明，该方法使用了多核苷酸激酶，不会导致mRNA的进一步降解。在小麦胚芽系统中，经酶促脱帽的mRNA在支持蛋白质合成方面与未处理的mRNA一样有效。在广泛的mRNA浓度范围内以及相当长的一段时间内都是如此。甲基化抑制剂S-腺苷同型半胱氨酸存在于孵育混合物中并不影响结果。数据表明，mRNA 5'端存在7-甲基鸟苷二磷酸残基并非真核系统翻译的必要条件。
