酶促去帽mRNA的末端标记
End labeling of enzymatically decapped mRNA.
作者信息
Efstratiadis A, Vournakis J N, Donis-Keller H, Chaconas G, Dougall D K, Kafatos F C
出版信息
Nucleic Acids Res. 1977 Dec;4(12):4165-74. doi: 10.1093/nar/4.12.4165.
Abstract
A method is presented for rapid and efficient 5' end labeling with 32P of capped mRNAs, by a series of three enzymatic reactions: the blocking nucleotide of the cap structure is removed by tobacco acid pyrophosphatase, and after dephosphorylation with alkaline phosphatase the 5' end is labeled with gamma-32-P-ATP and T4 polynucleotide kinase.
摘要
本文介绍了一种通过一系列三步酶促反应，用³²P对带帽mRNA进行快速高效5'端标记的方法：帽结构的封闭核苷酸被烟草酸焦磷酸酶去除，用碱性磷酸酶去磷酸化后，5'端用γ-³²P-ATP和T4多核苷酸激酶进行标记。
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