Ossai Chukwunalu O, Balogun Morufat O, Maroya Norbert G
Department of Crop Protection and Environmental Biology, University of Ibadan, Ibadan, Nigeria.
International Institute of Tropical Agriculture, IITA, Ibadan, Nigeria.
In Vitro Cell Dev Biol Plant. 2025;61(3):571-581. doi: 10.1007/s11627-023-10397-7. Epub 2023 Nov 27.
The primary goal of this study was to compare the multiplication rates of yam varieties propagated through organogenesis and somatic embryogenesis (SE). Callus was induced from axillary bud explants of three genotypes of (Asiedu, Ekiti2a, and Kpamyo) and two genotypes of (Swaswa and TDa2014) cultured in Murashige and Skoog (MS) medium containing 9.1 µM 2,4-dichlorophenoxylacetic acid and 5.4 µM naphthaleneacetic acid. Plantlets were regenerated in MS containing 4.4 µM benzylaminopurine and 34 µM uniconazole-P through SE. Single-node cuttings of the five genotypes were grown in MS for 8 wk organogenesis. The SE and organogenesis regenerants were acclimatized and potted in a 2 (propagation techniques (PTs)) × 5 (genotypes) factorial arranged in a completely randomized design ( = 10). The multiplication ratios (MR), number of tubers (NoT) of the SE, and organogenesis regenerants were collected and analyzed using ANOVA, and means were separated using DMRT ( ≤ 0.05). The SE and organogenesis MR ranged from 1:2 (TDa2014) to 1:8 (Asiedu) and 1:4 (Asiedu) to 1:5 (Ekiti2a and TDa2014), respectively. The NoT differed among genotypes, ranging from 1.15 ± 0.49 (Swaswa) to 2.45 ± 1.39 (Asiedu), and between PTs, ranging from 1.42 ± 0.70 (SE) to 1.86 ± 1.11 (organogenesis). The optimum propagation pathway was genotype-specific.
本研究的主要目标是比较通过器官发生和体细胞胚胎发生（SE）繁殖的山药品种的增殖率。从三种基因型（阿西杜、埃基蒂2a和卡姆约）和两种基因型（斯瓦斯瓦和TDa2014）的腋芽外植体诱导愈伤组织，将其培养在含有9.1µM 2,4 - 二氯苯氧乙酸和5.4µM萘乙酸的Murashige和Skoog（MS）培养基中。通过体细胞胚胎发生在含有4.4µM苄氨基嘌呤和34µM烯效唑 - P的MS培养基中再生植株。将这五个基因型的单节插条在MS中培养8周用于器官发生。将体细胞胚胎发生和器官发生的再生植株进行驯化，并按照2（繁殖技术（PTs））×5（基因型）析因设计种植在完全随机设计（n = 10）中。收集并使用方差分析（ANOVA）分析体细胞胚胎发生和器官发生再生植株的增殖率（MR）、块茎数量（NoT），并使用Duncan多重范围检验（DMRT）分离均值（P≤0.05）。体细胞胚胎发生和器官发生的增殖率分别为1:2（TDa2014）至1:8（阿西杜）和1:4（阿西杜）至1:5（埃基蒂2a和TDa2014）。块茎数量在基因型之间有所不同，范围从1.15±0.49（斯瓦斯瓦）到2.45±1.39（阿西杜），在繁殖技术之间也有所不同，范围从1.42±0.70（体细胞胚胎发生）到1.86±1.11（器官发生）。最佳繁殖途径具有基因型特异性。
