Xu Zhoujin, Yan Li, Yang Zhencong, Feng Xue, Fan Yuting, Wu Xuli
School of Public Health, Shenzhen University Medical School, Shenzhen University, Shenzhen, Guangdong Province 518060, PR China.
School of Basic Medical Sciences, Shenzhen University Medical School, Shenzhen University, Shenzhen, Guangdong Province 518060, PR China.
J Agric Food Chem. 2025 Aug 27;73(34):21598-21608. doi: 10.1021/acs.jafc.5c07402. Epub 2025 Aug 15.
Acesulfame potassium (AK), a widely used synthetic non-nutritive sweetener in low-calorie food and beverage products, has not been comprehensively investigated for its potential modulatory effects on food allergies. This study systematically investigated the therapeutic potential of AK using both an ovalbumin (OVA)-induced murine food allergy model and IgE/BSA-activated RBL-2H3 mast cells. experiments demonstrated that AK administration during OVA sensitization significantly alleviated allergic manifestations, showing a 78% reduction in diarrhea incidence and systemic anaphylactic scores compared with those of the OVA-sensitized controls. Serum biomarker analysis revealed that AK effectively suppressed the production of allergy-related mediators, including IgE, IgG1, histamine, and β-hexosaminidase. Histopathological examination indicated preserved duodenal architecture with increased villus height. Mechanistically, AK restored immune homeostasis by rebalancing Th1/Th2 and Th17/Treg responses while suppressing Th2- and Th17-associated cytokine production. experiments demonstrated that AK dose-dependently inhibited mast cell degranulation and suppressed the secretion of pro-inflammatory cytokines (IL-4, IL-6, and IL-13). Western blot analysis further revealed that AK significantly suppressed the MAPK signaling pathway in activated mast cells, with reductions of 60% in p-JNK, 57% in p-ERK, and 64% in p38 phosphorylation. These findings elucidate that AK exerts dual protective effects through both mast cell stabilization and systemic immunomodulation, proposing its potential incorporation into hypoallergenic diets. The study provides novel insights into food additives' capacity to regulate hypersensitivity reactions, with implications for developing functional foods targeting immune-mediated disorders.
乙酰磺胺酸钾（AK）是一种广泛应用于低热量食品和饮料产品中的合成非营养性甜味剂，其对食物过敏的潜在调节作用尚未得到全面研究。本研究使用卵清蛋白（OVA）诱导的小鼠食物过敏模型和IgE/BSA激活的RBL-2H3肥大细胞，系统地研究了AK的治疗潜力。实验表明，在OVA致敏期间给予AK可显著减轻过敏表现，与OVA致敏对照组相比，腹泻发生率和全身过敏评分降低了78%。血清生物标志物分析显示，AK有效抑制了包括IgE、IgG1、组胺和β-己糖胺酶在内的过敏相关介质的产生。组织病理学检查表明十二指肠结构保留，绒毛高度增加。从机制上讲，AK通过重新平衡Th1/Th2和Th17/Treg反应，同时抑制Th2和Th17相关细胞因子的产生，恢复了免疫稳态。实验表明，AK剂量依赖性地抑制肥大细胞脱颗粒，并抑制促炎细胞因子（IL-4、IL-6和IL-13）的分泌。蛋白质印迹分析进一步显示，AK显著抑制活化肥大细胞中的MAPK信号通路，p-JNK降低60%，p-ERK降低57%，p38磷酸化降低64%。这些发现阐明了AK通过肥大细胞稳定和全身免疫调节发挥双重保护作用，提出了将其纳入低敏饮食的可能性。该研究为食品添加剂调节超敏反应的能力提供了新的见解，对开发针对免疫介导疾病的功能性食品具有重要意义。
