Hashemzehi Mozhgan, Håkansson Helena, Carlsson Kvarnlöf Gunilla, Henriksson Gunnar, Sjöstrand Björn
Department of Engineering and Chemical Science, Karlstad University, Universitetsgatan 2, 65188 Karlstad, Sweden.
Wallenberg Wood Science Center, School of Engineering Sciences in Chemistry, Biotechnology and Health, Royal Institute of Technology, KTH, 100 44 Stockholm, Sweden.
Biomacromolecules. 2025 Sep 8;26(9):5581-5590. doi: 10.1021/acs.biomac.4c01819. Epub 2025 Aug 15.
In the manufacturing of cellulose derivatives, improving cellulose accessibility is essential for achieving a high product quality. In this study, endoglucanase enzyme treatment was applied prior to the cationization reaction to enhance the accessibility of hydroxyl groups for the production of cationized dialdehyde cellulose (CDAC). A range of enzyme dosages (0.09-45.00 ECU/g) was tested, and their effects on the swelling behavior and surface charge density of the final product were evaluated. The surface charge density of the ultimate cellulosic derivative confirmed its cationization and was proven to enhance the charge density of cationized dialdehyde cellulose (35% increase) compared to untreated pulp with enzyme. Additionally, the modified cellulose exhibited a significantly higher swelling capacity than regular pulps. These findings suggest that enzymatic pretreatment can enhance fiber reactivity and support a more sustainable and efficient production of cellulose-based derivatives, offering a promising potential for commercial applications.
在纤维素衍生物的制造过程中，提高纤维素的可及性对于获得高质量产品至关重要。在本研究中，在阳离子化反应之前应用内切葡聚糖酶处理，以提高羟基的可及性，用于生产阳离子化二醛纤维素（CDAC）。测试了一系列酶剂量（0.09 - 45.00 ECU/g），并评估了它们对最终产品溶胀行为和表面电荷密度的影响。最终纤维素衍生物的表面电荷密度证实了其阳离子化，并且与未经酶处理的纸浆相比，阳离子化二醛纤维素的电荷密度提高了（增加35%）。此外，改性纤维素表现出比普通纸浆显著更高的溶胀能力。这些发现表明，酶预处理可以提高纤维反应性，并支持更可持续和高效地生产纤维素基衍生物，具有良好的商业应用潜力。
