Lee Sangeun, Lee Jihyun, Ok Hangji, Ryu Eunbin, Koo Wonhee, Lee Yoon Hee, Pyo Jeasung, Lim Na Young, Kwon Chan Hyeok, Park Su Jeong, Jung Kikyung, Eom Yoonsook, Chon Younghoon, Song Min-Kyu, Hong Youngmin, Han Eunyoung
College of Pharmacy, Duksung Women's University, 33, Samyang-ro 144-gil, Dobong-gu, Seoul 01369, Republic of Korea.
Technical Research Center, Shimadzu Scientific Korea, 145, Gasan digital 1-ro, Geumcheon-gu, Seoul 08506, Republic of Korea.
J Pharm Biomed Anal. 2025 Dec 15;266:117096. doi: 10.1016/j.jpba.2025.117096. Epub 2025 Aug 8.
Polydrug use is a growing concern in the forensic field due to its high risk of overdose and its significant impact on physical and psychological health. Hence, the development of methods for simultaneously analyzing multiple drugs in blood has become increasingly essential recently. The aim of this study is to develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous detection of 195 drugs of abuse (DOAs), including amphetamines, opiates, cathinones, phencyclidines, synthetic cannabinoids, cocaine, and metabolites, in 100 μL of blood. Blood samples were extracted using liquid-liquid extraction (LLE) with acetonitrile containing 0.1 % trifluoroacetic acid, and multiple reaction monitoring (MRM) was employed for the detection of 195 drugs and metabolites in a single run. The method was validated over a concentration range of 1-100 ng/mL for all target compounds, showing good linearity with a coefficient of determination (R²) greater than 0.99 for 193 compounds. The limits of quantification (LOQs) of target analytes ranged 0.1-10 ng/mL. The method was successfully applied to 20 real blood samples, detecting 22 drugs of abuse and their metabolites, with concentrations ranging from 0.2 to 401.4 ng/mL. This developed method is expected to be highly applicable in the forensic or clinical field for the rapid detection of polydrug use.
由于多药滥用存在高过量风险且对身心健康有重大影响，其在法医领域日益受到关注。因此，近年来开发同时分析血液中多种药物的方法变得愈发重要。本研究的目的是开发一种液相色谱 - 串联质谱（LC-MS/MS）方法，用于在100μL血液中同时检测195种滥用药物（DOA），包括苯丙胺类、阿片类、卡西酮类、苯环己哌啶、合成大麻素、可卡因及其代谢物。血液样本采用含0.1%三氟乙酸的乙腈进行液 - 液萃取（LLE），并采用多反应监测（MRM）在一次运行中检测195种药物和代谢物。该方法在1 - 100 ng/mL的浓度范围内对所有目标化合物进行了验证，193种化合物的决定系数（R²）大于0.99，显示出良好的线性。目标分析物的定量限（LOQ）范围为0.1 - 10 ng/mL。该方法成功应用于20份实际血液样本，检测到22种滥用药物及其代谢物，浓度范围为0.2至401.4 ng/mL。这种开发的方法有望在法医或临床领域高度适用于快速检测多药滥用情况。
