青年运动员和成年运动员对急性运动和免疫挑战的不同炎症反应。

Differential inflammatory responses to acute exercise and immune challenge in young and master athletes.

作者信息

Minuzzi Luciele Guerra, De Souza Teixeira Alexandre Abilio, Figueiredo Caique, Dorneles Gilson, Branco Anna Cláudia Castelo, Silva Bruna Spolador de Alencar, Valenzuela Pedro L, Peres Alessandra, Lucia Alejandro, Sato Maria Notomi, Rosa Neto José Cesar, Krüger Karsten, Lira Fabio Santos

机构信息

Exercise and Immunometabolism Research Group, Postgraduation Program in Movement Sciences, Department of Physical Education, Universidade Estadual Paulista "Julio de Mesquita Filho", Sao Paulo, Brazil.

Department of Exercise Physiology and Sports Therapy, Institute of Sport Science, Justus-Liebig-University Giessen, Giessen, Germany.

出版信息

Front Immunol. 2025 Jul 31;16:1601405. doi: 10.3389/fimmu.2025.1601405. eCollection 2025.

DOI:10.3389/fimmu.2025.1601405

PMID:40821811

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12350127/

Abstract

BACKGROUND

Lifelong exercise is associated with beneficial immune adaptations, but the extent to which these adaptations manifest during an acute inflammatory challenge remains unclear. Therefore, we aimed to compare the inflammatory responses to ex vivo whole blood and peripheral blood mononuclear cells [PBMCs] cultures from young and master athletes, before and after a single bout of moderate-intensity exercise.

METHODS

Young (n=7; 22 ± 4 years) and master (n=12; 52 ± 9 years) female and male athletes with similar performance levels performed a 30-minute bout of moderate-intensity exercise. Blood samples were collected before and post-exercise to assess cytokine production in whole blood and PBMCs after stimulation with lipopolysaccharide [LPS] and a cocktail with phorbol 12-myristate 13-acetate [PMA] plus ionomycin.

RESULTS

In whole blood, LPS induced higher interleukin [IL]-6 release in both groups, with a greater increase in young athletes at post-exercise (p=0.014). Tumor necrosis factor [TNF]-α levels increased only in young athletes (p<0.0001). In PBMCs, master athletes showed lower LPS-induced TNF-α release, increasing only post-exercise (p<0.034), whereas young athletes responded at both baseline (p<0.001) and post-exercise (p=0.003). Under PMA/ionomycin stimulation, TNF-α (p<0.0001) and interferon (IFN)-γ (p=0.007) release increased only in young athletes, while IL-6 production decreased in young athletes at baseline (p=0.002) and post-exercise (p=0.003).

CONCLUSION

Young athletes exhibit a stronger cytokine response to ex vivo inflammatory stimuli, while master athletes demonstrate a more controlled and regulated inflammatory profile.

摘要

背景

终身运动与有益的免疫适应相关，但这些适应在急性炎症挑战期间的表现程度仍不清楚。因此，我们旨在比较年轻运动员和成年运动员在单次中等强度运动前后，对体外全血和外周血单个核细胞（PBMCs）培养物的炎症反应。

方法

具有相似运动水平的年轻（n = 7；22±4岁）和成年（n = 12；52±9岁）男女运动员进行了30分钟的中等强度运动。在运动前后采集血样，以评估用脂多糖（LPS）以及佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）加离子霉素的混合物刺激后全血和PBMCs中的细胞因子产生情况。

结果

在全血中，LPS诱导两组中白细胞介素（IL）-6释放增加，年轻运动员在运动后增加得更多（p = 0.014）。肿瘤坏死因子（TNF）-α水平仅在年轻运动员中升高（p < 0.0001）。在PBMCs中，成年运动员显示LPS诱导的TNF-α释放较低，仅在运动后增加（p < 0.034），而年轻运动员在基线（p < 0.001）和运动后（p = 0.003）均有反应。在PMA/离子霉素刺激下，TNF-α（p < 0.0001）和干扰素（IFN）-γ（p = 0.007）释放仅在年轻运动员中增加，而年轻运动员在基线（p = 0.002）和运动后（p = 0.003）IL-6产生减少。