Yang Xiaokun, Huang Mingshu, Chen Huan, Dai Jingtao, Chen Jiahao, Chen Kai, Zhou Jiannan, Li An, Li Ping
School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Medical University, Guangzhou, China.
Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou Medical University, Guangzhou, China.
Bioact Mater. 2025 Aug 8;54:1-33. doi: 10.1016/j.bioactmat.2025.07.047. eCollection 2025 Dec.
Biomaterials serve as bridges between synthetic structures and biological systems, facilitating interactions with bodily tissues and regulating cellular responses. However, the intricate mechanisms governing cell-material interactions within the body are not fully understood. To address these challenges, single-cell transcriptomics and spatial technologies have emerged as promising tools. This review introduces the concept of the 'Biomaterial-mediated Cell Atlas' and summarizes its recent advances in biomaterials research. Building upon the framework of the Human Cell Atlas, it can extend its scope to include biomaterial-mediated cellular responses. The principles and workflows of single-cell and spatial transcriptomics are then presented, emphasizing their potential to reveal cellular diversity and spatial arrangements affecting material-cell interactions. Furthermore, transcriptomics-driven approaches are highlighted for their potential in mapping biomaterial-cell communication pathways, which are essential for elucidating biological mechanisms and guiding material design. Challenges inherent to current methodologies and emerging frontiers in this interdisciplinary field are discussed.
生物材料充当合成结构与生物系统之间的桥梁，促进与身体组织的相互作用并调节细胞反应。然而，体内细胞与材料相互作用的复杂机制尚未完全了解。为应对这些挑战，单细胞转录组学和空间技术已成为有前景的工具。本综述介绍了“生物材料介导的细胞图谱”的概念，并总结了其在生物材料研究中的最新进展。基于人类细胞图谱的框架，它可以扩展其范围以包括生物材料介导的细胞反应。然后介绍了单细胞和空间转录组学的原理和工作流程，强调了它们揭示影响材料-细胞相互作用的细胞多样性和空间排列的潜力。此外，转录组学驱动的方法因其在绘制生物材料-细胞通讯途径方面的潜力而受到关注，这对于阐明生物学机制和指导材料设计至关重要。讨论了当前方法固有的挑战以及这一跨学科领域的新兴前沿。
