Dutca Laura M, Freed Emily F, Baserga Susan J
Molecular Biophysics & Biochemistry, Yale University, New Haven, Connecticut, Iowa City VA Health Care System, Iowa City, Iowa, United States.
Genetics, Yale School of Medicine, New Haven, Connecticut, Dept. of Physics, Colorado School of Mines, Golden, Colorado, United States.
MicroPubl Biol. 2025 Jun 27;2025. doi: 10.17912/micropub.biology.001593. eCollection 2025.
Maturation of the pre-ribosomal RNA (pre-rRNA) in eukaryotes involves a series of processing steps that remove transcribed spacer sequences to produce mature ribosomes. In many organisms two processing sites are located in the 5'external transcribed spacer (5'ETS), along with the site that defines the 5'-end of the 18S rRNA. However, the pre-rRNA processing site near the start site of transcription, known as A', has long been believed to be absent in the single-celled yeast, . Here we provide evidence that the A' pre-rRNA processing site is also present in the yeast 5'ETS, confirming conservation among single-celled and multicellular eukaryotes.
真核生物中前核糖体RNA（pre-rRNA）的成熟涉及一系列加工步骤，这些步骤会去除转录间隔序列以产生成熟核糖体。在许多生物体中，两个加工位点位于5'外部转录间隔区（5'ETS），以及定义18S rRNA 5'端的位点。然而，长期以来人们一直认为，转录起始位点附近的前rRNA加工位点（称为A'）在单细胞酵母中不存在。在这里，我们提供证据表明，A'前rRNA加工位点也存在于酵母5'ETS中，这证实了单细胞和多细胞真核生物之间的保守性。
MicroPubl Biol. 2025-6-27
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