Siegel E G, Bonfiglio J F, Ritschel W A, Fant W
Vet Hum Toxicol. 1985 Dec;27(6):503-5.
This study was designed to determine the effectiveness of clonidine (CL) and CL analogue, lofexidine (LF), in reducing the incidence and/or severity of convulsion associated with barbiturate withdrawal in mice. CL and LF have been shown to be useful in blocking opioid withdrawal symptoms in rodents and man. Some of the symptoms and possibly the mechanism(s) of the barbiturate withdrawal syndrome are similar to those of the opioid withdrawal syndrome. Therefore, we hypothesized that LF and CL may be efficacious in treating barbiturate withdrawal. The method of Belknap et al as modified by Blum et al for inducing barbiturate dependence in mice was used. Phenobarbital was thoroughly mixed with the animals' milled diet. After 10 days 45 pre-treated mice were divided into withdrawal, control, clonidine- and lofexidine-treated groups. Withdrawal scores were graded as 0 = no effect, 1 = tremor, 2 = wild running, 3 = tonic-clonic seizures, 4 = death, and were collected for each group every 5 hr for 2.5 days. The data in this model suggest that CL and LF offer no protective effect in treating barbiturate withdrawal-induced seizures in mice.
本研究旨在确定可乐定（CL）及其类似物洛非西定（LF）在降低小鼠巴比妥类药物戒断相关惊厥的发生率和/或严重程度方面的有效性。已证明CL和LF可有效阻断啮齿动物和人类的阿片类药物戒断症状。巴比妥类药物戒断综合征的一些症状以及可能的机制与阿片类药物戒断综合征相似。因此，我们假设LF和CL可能对治疗巴比妥类药物戒断有效。采用了由Blum等人修改的Belknap等人诱导小鼠巴比妥类药物依赖的方法。将苯巴比妥与动物的磨碎食物充分混合。10天后，将45只预处理小鼠分为戒断组、对照组、可乐定治疗组和洛非西定治疗组。戒断评分分为0 = 无影响，1 = 震颤，2 = 狂奔，3 = 强直阵挛性惊厥，4 = 死亡，在2.5天内每5小时收集每组的评分数据。该模型中的数据表明，CL和LF对治疗小鼠巴比妥类药物戒断引起的惊厥没有保护作用。
