Achan Beatrice, Wembabazi Abel, Luggya Tonny, Ebwongu Innocent Robert, Musinguzi Benson, Itabangi Herbert, Kajumbula Henry, Meya David
Department of Medical Microbiology, School of Biomedical Sciences, College of Health Sciences, Makerere University, Kampala, Uganda.
Department of Dentistry, School of Dentistry, College of Health Sciences, Makerere University, Kampala, Uganda.
PLoS One. 2025 Aug 18;20(8):e0329947. doi: 10.1371/journal.pone.0329947. eCollection 2025.
We aimed to determine the environmental sources of C. neoformans and C. gattii species complexes in Uganda.
One hundred fifty (n = 150) environmental specimens of chicken droppings, marabou stork bird droppings and eucalyptus tree barks in Uganda were examined phenotypically. The specimens were grown on Sabouraud dextrose agar and the colonies examined by India ink microscopy, urea hydrolysis and formation of blue colonies on chromogenic L-Canavanine glycine blue bromothymol blue agar of C. gattii species complex.
The prevalence of C. neoformans species complex was 4% (6/150). Of the positive environmental samples from which C. neoformans species complex were isolated; the predominant source was marabou stork droppings; 8% (2/25) followed by eucalyptus tree barks; 3.96% (4/101). However, there was no Cryptococcus gattii species complex; 0% (0/150).
In Uganda, the environmental sources of C. neoformans species complex are marabou stork droppings and eucalyptus tree barks, however, there seems to be no Cryptococcus gattii species complex.
我们旨在确定乌干达新型隐球菌和格特隐球菌复合群的环境来源。
对乌干达150份鸡粪、秃鹳粪便和桉树皮的环境标本进行表型检查。将标本接种在沙氏葡萄糖琼脂上，通过墨汁负染显微镜检查、尿素水解试验以及在格特隐球菌复合群的显色L-刀豆氨酸甘氨酸蓝溴麝香草酚蓝琼脂上形成蓝色菌落来检查菌落。
新型隐球菌复合群的患病率为4%（6/150）。在分离出新型隐球菌复合群的阳性环境样本中，主要来源是秃鹳粪便，占8%（2/25），其次是桉树皮，占3.96%（4/101）。然而，未发现格特隐球菌复合群，患病率为0%（0/150）。
在乌干达，新型隐球菌复合群的环境来源是秃鹳粪便和桉树皮，然而，似乎不存在格特隐球菌复合群。
