Yu Wei, Luo Huilong, Han Bo, Lin Sisi, Li Qian, Xue Rui, Tang Hui, Jia Xin, Wang Lu, Liu Jinyao
School of Chemistry and Chemical Engineering, School of Pharmacy, Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Shihezi University, Shihezi, Xinjiang, China.
Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Nat Commun. 2025 Aug 18;16(1):7691. doi: 10.1038/s41467-025-63110-0.
The mucosal barrier consisting of physicochemical, immune, and microbial components is the first line of defense against external stimuli. Breakdown of the mucosal barrier causes the occurrences of various diseases, while methods capable of multifacetedly restoring mucosal barrier functions have been rarely reported. Here, we describe the restoration of the physicochemical, immune, and microbial homeostasis of the mucosal barrier by in situ formation of a living-synthetic therapeutic coating (LSTC). Through metal-phenolic complexation and π-π stacking interactions, ethyl gallate can chelate Bi ions to form an adhesive coating on mucosal surfaces, which enables further hybridization with living bacteria. Due to the beneficial effects of Bi and ethyl gallate and the probiotic characteristic of carried bacteria, LSTC increases the barrier integrity, mitigates mucosal inflammation, and maintains normal homoeostasis of the microbiota. In two murine models of aerobic vaginitis and vaginal candidiasis, LSTC demonstrates the potency to alleviate vaginal pathological injury and decrease vaginal inflammatory infiltration.
由物理化学、免疫和微生物成分组成的黏膜屏障是抵御外部刺激的第一道防线。黏膜屏障的破坏会导致各种疾病的发生，而能够多方面恢复黏膜屏障功能的方法鲜有报道。在此，我们描述了通过原位形成一种生物合成治疗性涂层（LSTC）来恢复黏膜屏障的物理化学、免疫和微生物稳态。通过金属 - 酚络合和π - π堆积相互作用，没食子酸乙酯可以螯合铋离子，在黏膜表面形成一层粘附性涂层，这使得其能够与活菌进一步杂交。由于铋和没食子酸乙酯的有益作用以及携带细菌的益生菌特性，LSTC提高了屏障完整性，减轻了黏膜炎症，并维持了微生物群的正常稳态。在需氧性阴道炎和阴道念珠菌病的两种小鼠模型中，LSTC显示出减轻阴道病理损伤和减少阴道炎症浸润的效力。
