Introduction Tissue adhesives, such as 2-octyl cyanoacrylate, are widely used for tissue approximation and protection of surgical wounds during total knee arthroplasty (TKA). However, drying time for tissue adhesives can increase overall operating time and impede efficiency. Various methods may reduce the drying time of tissue adhesives. However, there is currently no clinical or in vivo data to support these findings. This study aims to investigate whether preheated 2-octyl cyanoacrylate (Dermabond™, Ethicon, Inc., Somerville, NJ, US) shortens drying time in robotic-assisted TKA. Methods This randomized pilot study included 40 patients undergoing TKA, and they were randomized into two groups: the intervention group received Dermabond™ preheated in a solution warmer device, while the control group received Dermabond™ at ambient temperature. Incision size, preparation, and drying times were recorded and compared. Comparison of drying time, preparation time, incision size, BMI, weight, height, age, surgery time, and gender between the preheated and control groups was conducted using the t-test, Levene's test, and the chi-squared test. Results Data from 39 patients were analyzed, as one intervention failed due to premature Dermabond™ drying in the preheated group. The drying time was reduced by an average of 26.8 seconds in the preheated group compared to the control group (p < 0.05). No significant differences were observed in preparation time or incision size between the groups. Conclusion Preheating Dermabond™ reduces drying time; however, the reduction does not provide substantial time savings to alter the current TKA workflow. Improvements in intraoperative efficiency may be better achieved by optimizing other aspects of the procedure.