滨蒿内酯的区域选择性O-去甲基化：大鼠肝脏细胞色素P-450同工酶之间的差异

Regioselective O-demethylation of scoparone: differentiation between rat liver cytochrome P-450 isozymes.

作者信息

Müller-Enoch D, Büttgen E, Nonnenmacher A

出版信息

Z Naturforsch C Biosci. 1985 Sep-Oct;40(9-10):682-4. doi: 10.1515/znc-1985-9-1017.

DOI:10.1515/znc-1985-9-1017

Abstract

The ratios of the scoparone O-demethylation products scopoletin to isoscopoletin were determined for reconstituted complexes of NADPH-P-450 reductase and each of four P-450 isozymes in a 2:1 molar ratio with a 1:1 mixture of [7-O-methyl-14C]- and [6-O-methyl-14C]-scoparone as substrate. The two phenobarbital inducible forms P-450PB-B and P-450PB-D have a 1:0.8 +/- 0.05 scopoletin to isoscopoletin ratio, and the two beta-naphthoflavone inducible forms P-450 beta NF-B and P-450 beta NF/ISF-G have ratios of 1:4.4 +/- 0.1 and 1:3.8 +/- 0.1, respectively. The scoparone-O-demethylation activities of the reconstituted preformed complexes of the four P-450 isozymes are given.

摘要

以[7 - O - 甲基 - ¹⁴C] - 和[6 - O - 甲基 - ¹⁴C] - 滨蒿内酯的1:1混合物为底物，测定了NADPH - P - 450还原酶与四种P - 450同工酶中每一种以2:1摩尔比重组的复合物的滨蒿内酯O - 去甲基化产物东莨菪内酯与异东莨菪内酯的比例。两种苯巴比妥诱导型P - 450PB - B和P - 450PB - D的东莨菪内酯与异东莨菪内酯比例为1:0.8±0.05，两种β - 萘黄酮诱导型P - 450βNF - B和P - 450βNF/ISF - G的比例分别为1:4.4±0.1和1:3.8±0.1。给出了四种P - 450同工酶重组预形成复合物的滨蒿内酯 - O - 去甲基化活性。