人垂体β-促脂素的免疫化学研究。

Immunochemical investigations of human pituitary beta-lipotropin.

作者信息

Rao A J, Li C H

出版信息

Int J Pept Protein Res. 1977;10(2):167-71. doi: 10.1111/j.1399-3011.1977.tb02791.x.

DOI:10.1111/j.1399-3011.1977.tb02791.x

Abstract

Antiserum to human beta-lipotropin has been raised in rabbits and characterized by gel double diffusion, quantitative precipitin, microcomplement fixation and biological neutralization tests. A sensitive radioimmunoassay for human beta-lipotropin has been developed. It was demonstrated that the antiserum shows specificity for this hormone. Human beta-melanotropin and human beta-endorphin showed only a minimal degree of cross-reaction, while ovine beta-lipotropin showed a partial but significant degree of cross-reactivity.

摘要

已在兔体内制备了抗人β-促脂素抗血清，并通过凝胶双向扩散、定量沉淀素、微量补体结合和生物中和试验进行了鉴定。已开发出一种用于人β-促脂素的灵敏放射免疫测定法。结果表明，该抗血清对这种激素具有特异性。人β-促黑素和人β-内啡肽仅表现出极低程度的交叉反应，而羊β-促脂素则表现出部分但显著程度的交叉反应性。