抗原-抗体反应激活一种新的血浆酶。

Activation of a new plasma enzyme by antigen-antibody reaction.

作者信息

Sumi H, Izumiya N, Muramatu M

出版信息

J Biochem. 1977 Jul;82(1):81-5. doi: 10.1093/oxfordjournals.jbchem.a131695.

DOI:10.1093/oxfordjournals.jbchem.a131695

Abstract

The change of enzyme activity in immunized rabbit plasma after addition of the homologous antigen was examined. The activities of N alpha-tosyl-L-arginine methyl ester (TAMe) and N alpha-tosyl-L-lysine methyl ester (TLMe) hydrolysis increased about 15 to 18 days after immunization. This increase was especially marked before the maximal rise of antibody content, and is thought to be related to the IgM antibody not to the IgG antibody. Enzyme activation was strongly inhibited by chelation of Ca2+ with 5 mM disodium ethylenediamine tetraacetate (EDTA), but not by other protease inhibitors, such as epsilon amino-caproic acid (epsilon-ACA), bovine lung kallikrein inhibitor (Trasylol) or soybean trypsin inhibitor (SBTI).

摘要

检测了加入同源抗原后免疫兔血浆中酶活性的变化。免疫后约15至18天，Nα-对甲苯磺酰-L-精氨酸甲酯（TAMe）和Nα-对甲苯磺酰-L-赖氨酸甲酯（TLMe）水解的活性增加。这种增加在抗体含量达到最大值之前尤为明显，并且被认为与IgM抗体有关，而非IgG抗体。用5 mM乙二胺四乙酸二钠（EDTA）螯合Ca2+可强烈抑制酶的激活，但其他蛋白酶抑制剂，如ε-氨基己酸（ε-ACA）、牛肺激肽释放酶抑制剂（抑肽酶）或大豆胰蛋白酶抑制剂（SBTI）则无此作用。