Ye Zhengqi, Qin Yongzhi, Zhang Haijun, Xian Meitian, Ye Hui, Cao Qingyun, Dong Zemin, Zhang Changming, Zuo Jianjun, Wang Weiwei
Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Poult Sci. 2025 Aug 8;104(11):105668. doi: 10.1016/j.psj.2025.105668.
This study aimed at investigating the influences of pectic oligosaccharide (POS) on growth performance, organ indexes and intestinal health in broilers exposed to aflatoxin B (AFB). In vitro experiment was conducted to assess reactive oxygen species (ROS)-scavenging ability of POS. In vivo experiment was then implemented by allocating 320 one-day-old yellow-feathered broilers to 4 groups (8 replicates/group), according to a 2 × 2 factorial arrangement with POS addition (0 or 400 mg/kg) and AFB exposure (with or without) as the two factors. All parameters were determined on d 21. The results revealed an in vitro ability of POS to scavenge ROS. AFB exposure tended to decrease (P < 0.10) average daily gain (ADG) and average daily feed intake (ADFI), as well as caused abnormities in liver, thymus and bursal indexes. It also elevated (P < 0.05) intestinal ROS and malondialdehyde contents but reduced (P < 0.05) intestinal catalase and glutathione peroxidase activities, as well as caused intestinal injuries including the decreasing trends (P < 0.10) of ileal villus height:crypt depth ratio and the relative mRNA expression of claudin-1 together with the increasing trend (P < 0.10) of serum diamine oxidase (DAO) activity. POS addition tended to elevate (P < 0.10) ADG and ADFI, as well as mitigated AFB-induced elevation (P < 0.10) in spleen index and reduction (P < 0.05) of bursal index. Moreover, POS addition tended to enhance (P < 0.10) ileal total antioxidant capacity in AFB-exposed broilers. Besides, POS addition lowered (P < 0.05) ROS level and increased (P < 0.05) glutathione content in ileum, as well as showed a tendency (P < 0.10) to elevate ileal claudin-1 expression and decrease serum DAO activity of broilers regardless of AFB exposure. Collectively, supplemental POS benefited growth performance and organ indexes of broilers exposed to AFB, which could attribute to its ability to ameliorate intestinal redox state and integrity. These findings underscore a potential of POS to attenuate the detriments of AFB contamination in diets.
本研究旨在探究果胶寡糖（POS）对暴露于黄曲霉毒素B（AFB）的肉鸡生长性能、器官指数及肠道健康的影响。进行体外实验以评估POS清除活性氧（ROS）的能力。随后进行体内实验，将320只1日龄黄羽肉鸡按2×2析因设计分为4组（每组8个重复），两个因素分别为POS添加量（0或400 mg/kg）和AFB暴露情况（有或无）。所有参数均在第21天测定。结果显示POS在体外具有清除ROS的能力。AFB暴露使平均日增重（ADG）和平均日采食量（ADFI）有降低趋势（P<0.10），并导致肝脏、胸腺和法氏囊指数异常。它还使肠道ROS和丙二醛含量升高（P<0.05），但降低了肠道过氧化氢酶和谷胱甘肽过氧化物酶活性（P<0.05），并造成肠道损伤，包括回肠绒毛高度与隐窝深度比值呈下降趋势（P<0.10）、紧密连接蛋白1（claudin-1）的相对mRNA表达下降以及血清二胺氧化酶（DAO）活性呈上升趋势（P<0.10）。添加POS使ADG和ADFI有升高趋势（P<0.10），并减轻了AFB引起的脾脏指数升高（P<0.10）和法氏囊指数降低（P<0.05）。此外，添加POS使AFB暴露肉鸡的回肠总抗氧化能力有增强趋势（P<0.10）。此外，无论AFB暴露情况如何，添加POS均降低了回肠ROS水平（P<0.05）并增加了谷胱甘肽含量（P<0.05），还使肉鸡回肠claudin-1表达有升高趋势（P<0.10）以及血清DAO活性降低。总体而言，补充POS有利于暴露于AFB的肉鸡的生长性能和器官指数，这可能归因于其改善肠道氧化还原状态和完整性的能力。这些发现凸显了POS减轻日粮中AFB污染危害的潜力。
