Kooistra L, Splitter G A, Albrecht R M
Am J Vet Res. 1985 Dec;46(12):2626-33.
Feline monocytes and neutrophils functioned as effector cells in antibody-dependent cellular cytotoxicity (ADCC) against antibody-coated chicken erythrocytes. Using light microscopy, effector cell populations were identified in effector-target cell interactions, with further characterization of these identical individual effector cells by histochemical evaluations and scanning electron microscopy. Monocytes and neutrophils, but not lymphocytes, were observed attacking target cells. Carbonyl iron depletion of monocytes and neutrophils from peripheral blood leukocytes caused a marked reduction from a mean of 62% to 3.6% lysis in ADCC as measured by a 4-hour 51Cr release assay. Effector cells functioning in the ADCC reaction were visualized, using sequential analysis and light microscopy, histochemistry, and scanning electron microscopy.
猫单核细胞和中性粒细胞在针对抗体包被的鸡红细胞的抗体依赖性细胞毒性（ADCC）中作为效应细胞发挥作用。通过光学显微镜，在效应细胞与靶细胞的相互作用中识别出效应细胞群体，并通过组织化学评估和扫描电子显微镜对这些相同的单个效应细胞进行进一步表征。观察到单核细胞和中性粒细胞而非淋巴细胞攻击靶细胞。通过4小时的51Cr释放试验测定，外周血白细胞中单核细胞和中性粒细胞的羰基铁耗竭导致ADCC中平均裂解率从62%显著降低至3.6%。使用序列分析、光学显微镜、组织化学和扫描电子显微镜对在ADCC反应中发挥作用的效应细胞进行了可视化观察。
