Miao Xiaoyu, Cao Henglong, Chen Jin, Gao Yang, Li Jiayi, Zhang Bin, Nie Min, Yu Miao
Department of Periodontics, School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou Medical University, Guangzhou 510182, China.
Department of Orthodontic, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou 510182, China.
Curr Res Microb Sci. 2025 Aug 8;9:100455. doi: 10.1016/j.crmicr.2025.100455. eCollection 2025.
Dental caries occur primarily due to the formation of excessive dental plaque biofilm on teeth surfaces. This study aims to analyze the supragingival microbial profiles of permanent teeth in caries-free and caries-active adolescents with fixed orthodontic appliances and to identify bacterial biomarkers that may be indicative of caries activity in specific population.
Twelve-year-old adolescents treated with fixed orthodontic appliances were selected as the study population, with 5 caries-free and 5 caries-active subjects. Supragingival plaque samples were collected from the first permanent molars for the 16S rRNA high-throughput sequencing analysis of the V3-V4 region.
There was no significant difference in alpha diversity and beta diversity between the caries-free and caries-active groups. LEfSe analysis showed that differentially abundant genera such as (), and species in the caries-free group, and (), , and species in the caries-active group, could be identified as potential biomarkers ( < 0.05). Co-occurrence network analysis revealed significant synergistic relationships among bacteria, with spp displaying the strongest associations with other genera.
Increases in and bacteria from the genera and were strongly associated with the caries-active status of adolescents undergoing orthodontic treatment with fixed appliances. While increases in and were linked to caries-free status. These findings highlight the importance of managing the oral microbiome in adolescents with fixed orthodontic appliances, as certain bacterial species can serve as indicators of caries risk.
龋齿主要是由于牙齿表面形成过多的牙菌斑生物膜所致。本研究旨在分析佩戴固定正畸矫治器的无龋和患龋青少年恒牙的龈上微生物谱，并确定可能指示特定人群龋病活动的细菌生物标志物。
选取接受固定正畸矫治器治疗的12岁青少年作为研究对象，其中无龋受试者5名，患龋受试者5名。从第一恒磨牙采集龈上菌斑样本，进行V3-V4区域的16S rRNA高通量测序分析。
无龋组和患龋组之间的α多样性和β多样性无显著差异。线性判别分析效应大小（LEfSe）分析表明，无龋组中差异丰富的菌属如（）、和种，以及患龋组中的（）、和种，可被确定为潜在生物标志物（<0.05）。共现网络分析揭示了细菌之间存在显著的协同关系，其中spp与其他菌属的关联最强。
属和属中的和细菌增加与接受固定矫治器正畸治疗的青少年的患龋状态密切相关。而和的增加与无龋状态有关。这些发现凸显了管理佩戴固定正畸矫治器青少年口腔微生物群的重要性，因为某些细菌物种可作为龋病风险的指标。
