Singh Sanju Kumari, Srivastav Amit Kumar, Chaurasiya Sunaina, Patel Sunita, Kumar Umesh, Kulhari Hitesh
School of Life Sciences, Central University of Gujarat, Gandhinagar, Gujarat, India.
School of Nano sciences, Central University of Gujarat, Gandhinagar, Gujarat, India.
Front Drug Deliv. 2025 Aug 13;5:1623317. doi: 10.3389/fddev.2025.1623317. eCollection 2025.
Colorectal cancer is among the most redundant cancer of the gastrointestinal tract, with its burden expected to rise 60% by 2030. Morin hydrate (MH) is a bioflavonoid with anticancer attributes. However, the implementation of MH is limited due to its hydrophobic properties, along with poor stability and bioavailability. Protein-based nanoparticle may encapsulate the drug and this complex can enhance the drug efficacy and delivery to colorectal carcinoma cells. To investigate the molecular interactions between BSA and MH, the Lamarckian genetic approach was used. In the current study, we prepared BSA encapsulated MH nanoparticles by desolvation method. The characterization of the nanoparticles was done by XRD, DSC, TGA and FTIR was performed to corroborate the results. MHNPs were spherical with a particle size of 90 nm determined by TEM and a zeta potential of -11 ± 5.90 mV. BSA nanoparticles improve the thermal stability and sustained release profile of Morin Hydrate, enabling its application as a phytochemical-based anticancer nanocarrier. The antioxidant test of MHNPs showed higher radical scavenging ability than MH. Additionally, our release investigations show that drug release occurs from the matrix of the nanoformulation to reach the target site efficiently. An increase in the anticancer potential was shown by an cytotoxicity assay in comparison to MH. These data suggest that MH was successfully encapsulated and enhanced solubility, resulting in greater bioavailability.
结直肠癌是胃肠道中最为常见的癌症之一，预计到2030年其负担将增加60%。桑色素水合物（MH）是一种具有抗癌特性的生物类黄酮。然而，由于其疏水性以及稳定性和生物利用度较差，MH的应用受到限制。基于蛋白质的纳米颗粒可以包裹药物，这种复合物可以提高药物疗效并将其递送至结肠癌细胞。为了研究牛血清白蛋白（BSA）与MH之间的分子相互作用，采用了拉马克遗传算法。在本研究中，我们通过去溶剂化法制备了包裹有MH的BSA纳米颗粒。通过X射线衍射（XRD）、差示扫描量热法（DSC）、热重分析法（TGA）对纳米颗粒进行表征，并进行傅里叶变换红外光谱（FTIR）分析以证实结果。通过透射电子显微镜（TEM）测定，MH纳米颗粒呈球形，粒径为90 nm，zeta电位为-11±5.90 mV。BSA纳米颗粒提高了桑色素水合物的热稳定性和缓释特性，使其能够作为一种基于植物化学的抗癌纳米载体应用。MH纳米颗粒的抗氧化试验表明其自由基清除能力高于MH。此外，我们的释放研究表明，药物从纳米制剂基质中释放出来，能够有效地到达靶位点。与MH相比，细胞毒性试验显示抗癌潜力有所增加。这些数据表明，MH被成功包裹，溶解度提高，从而具有更高的生物利用度。
