Therapeutic effect of fecal microbiota transplantation on hyperuricemia mice by improving gut microbiota.

OBJECTIVE

The primary objective of this study was to assess the impact of fecal microbiota transplantation (FMT) on serum biochemical parameters, renal injury, and gut microbiota in hyperuricemia (HUA) mice.

METHODS

Six-week-old male C57BL/6 J mice were given a high-purine diet and potassium oxonate injections to induce HUA, followed by a two-week FMT treatment. Regular body weight checks, serum biochemical analyses, and fecal sampling for 16S rRNA gene sequencing were conducted to evaluate the treatment's impact on gut microbiota.

RESULTS

The model group showed significant increases in uric acid (UA), creatinine (Cr), blood urea nitrogen (BUN) levels, and increased xanthine oxidase (XOD) activity compared to controls ( < 0.05). FMT treatment effectively reduced these levels and XOD activity ( < 0.05). At the genus level, specific taxa like and were less abundant, while and were more abundant in the model group. Following FMT, gut microbiota composition returned to near-normal levels, with significant differences from the model group ( < 0.05).

CONCLUSION

This study demonstrates that FMT holds therapeutic potential for HUA mice by reducing UA levels, alleviating renal damage, and restoring gut microbiota balance.