Yuan Songjian, Jia Wenting, Liu Xiaomei, Liu Ruzhen, Cao Man, Wu Yuting, Li Yuantao, Xu Wei, Xiao Chuanxing, Hong Zhenqiang, Zhang Bangzhou
Fujian University of Traditional Chinese Medicine, Fuzhou, China.
Xiamen Treatgut Biotechnology Co. Ltd., Xiamen, China.
Front Microbiol. 2025 Aug 5;16:1599107. doi: 10.3389/fmicb.2025.1599107. eCollection 2025.
The primary objective of this study was to assess the impact of fecal microbiota transplantation (FMT) on serum biochemical parameters, renal injury, and gut microbiota in hyperuricemia (HUA) mice.
Six-week-old male C57BL/6 J mice were given a high-purine diet and potassium oxonate injections to induce HUA, followed by a two-week FMT treatment. Regular body weight checks, serum biochemical analyses, and fecal sampling for 16S rRNA gene sequencing were conducted to evaluate the treatment's impact on gut microbiota.
The model group showed significant increases in uric acid (UA), creatinine (Cr), blood urea nitrogen (BUN) levels, and increased xanthine oxidase (XOD) activity compared to controls ( < 0.05). FMT treatment effectively reduced these levels and XOD activity ( < 0.05). At the genus level, specific taxa like and were less abundant, while and were more abundant in the model group. Following FMT, gut microbiota composition returned to near-normal levels, with significant differences from the model group ( < 0.05).
This study demonstrates that FMT holds therapeutic potential for HUA mice by reducing UA levels, alleviating renal damage, and restoring gut microbiota balance.
本研究的主要目的是评估粪便微生物群移植（FMT）对高尿酸血症（HUA）小鼠血清生化参数、肾损伤和肠道微生物群的影响。
给6周龄雄性C57BL/6 J小鼠喂食高嘌呤饮食并注射氧嗪酸钾以诱导HUA，随后进行为期两周的FMT治疗。定期检查体重、进行血清生化分析，并采集粪便样本进行16S rRNA基因测序，以评估该治疗对肠道微生物群的影响。
与对照组相比，模型组的尿酸（UA）、肌酐（Cr）、血尿素氮（BUN）水平显著升高，黄嘌呤氧化酶（XOD）活性增加（<0.05）。FMT治疗有效降低了这些水平和XOD活性（<0.05）。在属水平上，模型组中特定的分类群如 和 丰度较低，而 和 丰度较高。FMT后，肠道微生物群组成恢复到接近正常水平，与模型组有显著差异（<0.05）。
本研究表明，FMT通过降低UA水平、减轻肾损伤和恢复肠道微生物群平衡，对HUA小鼠具有治疗潜力。
