Stobart A K, Stymne S
Biochem J. 1985 Nov 15;232(1):217-21. doi: 10.1042/bj2320217.
Microsomal preparations from the developing cotyledons of safflower (Carthamus tinctorius) catalyse the acylation of sn-glycerol 3-phosphate in the presence of acyl-CoA. Under these conditions the radioactive glycerol in sn-glycerol 3-phosphate accumulates in phosphatidic acid, phosphatidylcholine, diacyl- and tri-acylglycerol. The incorporation of glycerol into phosphatidylcholine is via diacylglycerol and probably involves a cholinephosphotransferase. The results show that the glycerol moiety and the acyl components in phosphatidylcholine exchange with the diacylglycerol during the biosynthesis of diacylglycerol from phosphatidic acid. The continuous reversible transfer of diacylglycerol with phosphatidylcholine, which operates during active triacylglycerol synthesis, will control in part the polyunsaturated-fatty-acid quality of the final seed oil.
来自红花(Carthamus tinctorius)发育中的子叶的微粒体制剂在酰基辅酶A存在的情况下催化sn-甘油3-磷酸的酰化反应。在这些条件下,sn-甘油3-磷酸中的放射性甘油积累在磷脂酸、磷脂酰胆碱、二酰基甘油和三酰基甘油中。甘油掺入磷脂酰胆碱是通过二酰基甘油,可能涉及胆碱磷酸转移酶。结果表明,在由磷脂酸生物合成二酰基甘油的过程中,磷脂酰胆碱中的甘油部分和酰基成分与二酰基甘油发生交换。在活跃的三酰基甘油合成过程中起作用的二酰基甘油与磷脂酰胆碱的持续可逆转移,将部分控制最终种子油的多不饱和脂肪酸质量。