Abdulmuttaleb Nabaa Azhar, Oliwi Nasir Abdelhameed Abdelkhaliq, Alkubaisy Sami Awad, Mohsein Osama Akram
Department of Clinical Laboratories, College of Applied Medical Sciences, University of Kerbala, Iraq.
Al Iraqia University, College of Dentistry, Iraq.
Rep Biochem Mol Biol. 2025 Jan;13(4):525-539. doi: 10.61186/rbmb.13.4.525.
Apelin is a naturally produced ligand for G protein-linked receptors derived from a 77-amino acid pre-propeptide. The effect of apelin on the development of cardiovascular diseases and the relationship between the apelin gene and the apelin receptor.
The case-control study included 100 participants of people suffering from cardiovascular diseases. Samples were collected from patients hospitalized at the Nasiriyah Heart Center between November 10, 2023, and February 15, 2024. The study also included 50 healthy people who did not suffer from cardiovascular disease. The lipid profile was measured by spectrophotometer, and the Apelin level was measured by enzyme-linked immunosorbent assay. Four single nucleotide polymorphisms for Apelin (APLN) and Apelin receptor (APLNR) were chosen, and Sanger sequencing was used to genotype them accurately.
The findings indicated that there was no statistically significant difference in age between the two groups. Upon comparing the age demographics of the two groups in the study, the results indicated a lack of statistical significance in the levels of APLN or the lipid profile, despite the case group exhibiting markedly elevated Apelin and lipid levels compared to the control group. After multiple test adjustments (P < 0.05), neither the APLN rs2235310T allele nor the APLNR rs9943582 allele demonstrated an association with an elevated risk of coronary heart disease.
The investigation revealed no significant age variations or genetic correlations associated with CHD risk. However, rather than age or genetic differences, elevated apelin and cholesterol levels in the case group indicate these factors as primary contributors to cardiovascular risk.
Apelin是一种天然产生的G蛋白偶联受体配体，来源于一种77个氨基酸的前原肽。Apelin对心血管疾病发展的影响以及Apelin基因与Apelin受体之间的关系。
病例对照研究纳入了100名心血管疾病患者。样本于2023年11月10日至2024年2月15日期间从纳西里耶心脏中心住院患者中采集。该研究还包括50名无心血管疾病的健康人。用分光光度计测量血脂水平，用酶联免疫吸附测定法测量Apelin水平。选择了Apelin（APLN）和Apelin受体（APLNR）的四个单核苷酸多态性，并使用桑格测序对其进行准确基因分型。
结果表明，两组之间年龄无统计学显著差异。在比较研究中两组的年龄人口统计学数据时，结果表明APLN水平或血脂水平无统计学显著差异，尽管病例组的Apelin和血脂水平明显高于对照组。经过多次检验调整（P<0.05）后，APLN rs2235310T等位基因和APLNR rs9943582等位基因均未显示与冠心病风险升高有关。
调查显示，与冠心病风险相关的年龄差异或基因相关性不显著。然而，病例组中Apelin和胆固醇水平升高表明，这些因素而非年龄或基因差异是心血管风险的主要促成因素，而非年龄或基因差异。
