沃顿胶间充质基质细胞衍生的细胞外囊泡在体外3D培养系统的炎症应激下减轻椎间盘退变
Wharton's Jelly Mesenchymal Stromal Cell-Derived Extracellular Vesicles Attenuate Intervertebral Disc Degeneration Under Inflammatory Stress in an In Vitro 3D Culture System.
作者信息
Tilotta Veronica, Vadalà Gianluca, Di Giacomo Giuseppina, Ambrosio Luca, Cicione Claudia, Russo Fabrizio, Darinskas Adas, Papalia Rocco, Denaro Vincenzo
机构信息
Laboratory for Regenerative Orthopaedics, Operative Research Unit of Orthopaedic and Trauma Surgery Fondazione Policlinico Universitario Campus Bio-Medico Rome Italy.
Research Unit of Orthopaedic and Trauma Surgery, Departmental Faculty of Medicine and Surgery Università Campus Bio-Medico di Roma Rome Italy.
出版信息
JOR Spine. 2025 Aug 20;8(3):e70106. doi: 10.1002/jsp2.70106. eCollection 2025 Sep.
This study explores the therapeutic potential of extracellular vesicles (EVs) derived from Wharton's Jelly mesenchymal stem cells in an in vitro 3D model of intervertebral disc degeneration under inflammatory stress. The treatment with WJ-MSC-EVs enhanced nucleus pulposus cell proliferation, viability, and extracellular matrix synthesis while reducing oxidative stress and catabolic gene expression. These results support the promise of WJ-MSC-EVs as a novel, cell-free strategy for disc regeneration in inflammatory conditions.
本研究在炎症应激下的体外椎间盘退变三维模型中，探索了源自华通氏胶间充质干细胞的细胞外囊泡（EVs）的治疗潜力。用WJ-MSC-EVs进行治疗可增强髓核细胞增殖、活力和细胞外基质合成，同时减少氧化应激和分解代谢基因表达。这些结果支持了WJ-MSC-EVs作为炎症条件下椎间盘再生的一种新型无细胞策略的前景。
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