Kuroki Masashi, Kawaura Ryo, Tomita Hiroyuki, Shibata Hirofumi, Ohashi Toshimitsu, Ishikawa Tomohiko, Okada Hideshi, Hara Akira, Ogawa Takenori
Department of Otolaryngology-Head and Neck Surgery, Gifu University Graduate School of Medicine, Gifu, Japan.
Department of Tumor Pathology, Gifu University Graduate School of Medicine, Gifu, Japan.
J Pathol Clin Res. 2025 Sep;11(5):e70042. doi: 10.1002/2056-4538.70042.
Despite the use of targeted drugs for human epidermal growth factor receptor type 2 (HER2)-positive salivary duct carcinoma (SDC) treatment, the overall prognosis for SDC remains poor. In this study, we aimed to investigate whether the glycocalyx of SDC cells serves as a potential cell surface marker. To understand the complex structure of the glycocalyx of salivary gland tumors, we used a lectin with glycan-specific binding properties. Lectin staining was performed for five types of common salivary gland tumors. We determined the expression of the transmembrane glycoprotein mucin 1 with Tn antigen (GalNAc), Tn-MUC1, using 20 clinical SDC specimens. Vicia villosa lectin (VVL) was not stained in the vascular endothelium but specifically stained in the SDC tumor cells. GalNAc, to which VVL binds, formed Tn-MUC1 via glycosylation with N-acetylgalactosaminyltransferases (GALNTs). GALNT7 was highly expressed in SDC. Analysis of clinical SDC specimens revealed that Tn-MUC1 was also positive in the SDC tumor cells, suggesting its potential as a cell surface target for SDC.
尽管使用靶向药物治疗人表皮生长因子受体2（HER2）阳性涎腺导管癌（SDC），但SDC的总体预后仍然很差。在本研究中，我们旨在调查SDC细胞的糖萼是否作为一种潜在的细胞表面标志物。为了解涎腺肿瘤糖萼的复杂结构，我们使用了具有聚糖特异性结合特性的凝集素。对五种常见涎腺肿瘤进行凝集素染色。我们使用20例临床SDC标本确定了具有Tn抗原（GalNAc）的跨膜糖蛋白粘蛋白1即Tn-MUC1的表达。绒毛豌豆凝集素（VVL）在血管内皮中未染色，但在SDC肿瘤细胞中特异性染色。VVL结合的GalNAc通过与N-乙酰半乳糖胺基转移酶（GALNTs）糖基化形成Tn-MUC1。GALNT7在SDC中高表达。对临床SDC标本的分析显示，Tn-MUC1在SDC肿瘤细胞中也呈阳性，表明其作为SDC细胞表面靶点的潜力。
