Yoon Hyun-Joo, Park Ji-Young, Min Su-Jin, Lee Eun-Soo, Lee Na-Kyoung, Paik Hyun-Dong
Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, Republic of Korea.
Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, Republic of Korea.
Arch Biochem Biophys. 2025 Nov;773:110597. doi: 10.1016/j.abb.2025.110597. Epub 2025 Aug 20.
The yellow flower of the Asteraceae family, Inula britannica (IB) of have been used as a medicinal herb since ancient times. Modern studies have widely known the anti-inflammatory effects and antioxidant effects of IB. Therefore, this study aimed to analyze the neuroprotective effects of IB extract based on its antioxidant and anti-inflammatory effects and to assess its potential for development as a functional material. After preparing the extract of IB based on the solvent with water and 70 % ethyl alcohol (EtOH), the phytochemical components were identified. The effects of the IB extracts on the Nrf2-Keap1 signaling pathway, NF-κB signaling pathway, and Bax/Bcl-2 ratio were investigated in HO-induced SH-SY5Y cells using reverse transcription-polymerase chain reaction and western blotting. Both IB extracts activated the representative antioxidant signaling pathway Nrf2-Keap1 in a concentration-dependent manner. In the inflammation-related NF-κB signaling pathway, the expression of each factor was inhibited, and the Bax/Bcl-2 ratio, which is related to apoptosis, decreased upon IB extract treatment. Phytochemical analysis using liquid chromatography-tandem mass spectrometry identified polyphenols and flavonoids as the primary active compounds in IB. These findings indicate that IB extract has potential as a functional material for the treatment of neurodegenerative diseases.
菊科植物旋覆花（Inula britannica，IB）的黄色花朵自古以来就被用作草药。现代研究已广泛知晓旋覆花的抗炎作用和抗氧化作用。因此，本研究旨在基于其抗氧化和抗炎作用分析旋覆花提取物的神经保护作用，并评估其作为功能性材料的开发潜力。在用蒸馏水和70%乙醇（EtOH）作为溶剂制备旋覆花提取物后，对其植物化学成分进行了鉴定。使用逆转录-聚合酶链反应和蛋白质免疫印迹法，在过氧化氢（HO）诱导的SH-SY5Y细胞中研究了旋覆花提取物对Nrf2-Keap1信号通路、NF-κB信号通路以及Bax/Bcl-2比值的影响。两种旋覆花提取物均以浓度依赖性方式激活了代表性抗氧化信号通路Nrf2-Keap1。在与炎症相关的NF-κB信号通路中，各因子的表达受到抑制，并且在旋覆花提取物处理后，与细胞凋亡相关的Bax/Bcl-2比值降低。使用液相色谱-串联质谱法进行的植物化学分析确定多酚和黄酮类化合物是旋覆花中的主要活性成分。这些发现表明，旋覆花提取物具有作为治疗神经退行性疾病功能性材料的潜力。
