通过阴离子交换高效液相色谱法纯化鸟苷含量高的寡脱氧核糖核苷酸。
The purification of oligodeoxyribonucleotides with a high guanosine content by anion exchange HPLC.
作者信息
Coomber B A
出版信息
Biochimie. 1985 Jul-Aug;67(7-8):797-800. doi: 10.1016/s0300-9084(85)80170-x.
PMID:4084601
Abstract
Anion exchange HPLC purification of synthetic oligonucleotides with a high guanosine content is difficult. This study has demonstrated that these sequences can be successfully purified by anion exchange HPLC using standard conditions provided that the base protecting groups (isobutyryl[ib] and benzoyl[bz]) are retained on the otherwise deprotected oligomer.
摘要
对具有高鸟苷含量的合成寡核苷酸进行阴离子交换高效液相色谱纯化很困难。本研究表明，只要在其他方面已脱保护的寡聚物上保留碱基保护基团（异丁酰基[ib]和苯甲酰基[bz]），这些序列就可以使用标准条件通过阴离子交换高效液相色谱成功纯化。
Zotero 插件