寡脱氧核糖核苷酸化学合成中分离技术的一些改进。
Some improvements in the separation technique for the chemical syntheses of oligodeoxyribonucleotides.
作者信息
Tanaka H, Muraki M, Honda K, Nakazato S
出版信息
Nucleic Acids Symp Ser. 1982(11):73-6.
PMID:7183974
Abstract
Some improvements have been achieved for the separation technique in the syntheses of oligodeoxyribonucleotides by a solid phase phosphotriester method; (1) reuse of the protected nucleotides recovered from the reaction mixture by a preparative GPC. (2) a new weak anion exchange column, HPLC-DEAE, was used to estimate the size of the deblocked oligomers.
摘要
通过固相磷酸三酯法合成寡聚脱氧核糖核苷酸时，分离技术已取得了一些改进：（1）通过制备型凝胶渗透色谱法（GPC）回收反应混合物中得到的受保护核苷酸并重复利用。（2）使用一种新型弱阴离子交换柱HPLC-DEAE来评估脱保护寡聚物的大小。
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