芜菁黄花叶病毒核糖核酸的圆二色性研究

A circular dichroism study of the Turnip yellow mosaic virus-RNA.

作者信息

Katouzian-Safadi M, Charlier M, Maurizot J C

出版信息

Biochimie. 1985 Sep;67(9):1007-13. doi: 10.1016/s0300-9084(85)80295-9.

Abstract

We examined the circular dichroism spectra of intact Turnip yellow mosaic virus, freezed/thawed virus, empty capsid particles, and phenol extracted RNA. The circular dichroism signal of the empty capsid was found to contribute for less than 1% to the circular dichroism of the virus. Differences in the circular dichroism spectra indicate that TYMV-RNA exhibits different conformations when it is in situ in the virus, when it has been ejected by freezing/thawing and when it has been phenol extracted. Increase of the ionic strength up to 0.1 M NaCl led to conformational change of the RNA either freezed/thawed ejected or phenol extracted but not in situ in the capsid. Addition of spermidine (3 mM) induced a conformational change only for the phenol extracted RNA. These results are discussed with respect to the origin of the various conformational states of viral RNA.

摘要

我们检测了完整的芜菁黄花叶病毒、冻融病毒、空衣壳颗粒和苯酚抽提RNA的圆二色光谱。发现空衣壳的圆二色信号对病毒圆二色性的贡献小于1%。圆二色光谱的差异表明，芜菁黄花叶病毒RNA（TYMV-RNA）在病毒原位、经冻融释放后以及经苯酚抽提后呈现出不同的构象。将离子强度增加至0.1 M NaCl会导致经冻融释放或苯酚抽提的RNA发生构象变化，但衣壳内原位的RNA不会。添加亚精胺（3 mM）仅会使经苯酚抽提的RNA发生构象变化。我们针对病毒RNA各种构象状态的起源对这些结果进行了讨论。