Murphy W M
Biomaterials. 1985 Nov;6(6):427-30. doi: 10.1016/0142-9612(85)90106-1.
Calvaria from 6-day old mice labelled 4 days previously with 45CaCl2 were divided into test and control halves and each half cultured separately in vitro. Eluents from four endodontic materials, endomethasone, zinc oxide/eugenol, AH26 and gutta-percha were added separately to the culture medium of each test half. After 24 and 48 h culturing periods, the 45CaCl2 in the media and calvaria was measured by a standard liquid scintillation counting technique and a resorption ratio between test and control halves was computed. This ratio, based on cell mediated resorption, was an indication of toxicity of soluble components of each endodontic material. In accordance with the literature, endomethasone was found, with our method, to be the most toxic and gutta-percha the least toxic of the materials tested. This shows that our model can be used to test the toxicity of other biomaterials to bone cells in vitro.
取4天前用45CaCl2标记的6日龄小鼠的头盖骨，分成试验组和对照组两半，每半分别在体外培养。将四种牙髓材料（地塞米松、氧化锌/丁香酚、AH26和牙胶）的洗脱液分别加入到每个试验组半块头盖骨的培养基中。培养24小时和48小时后，采用标准液体闪烁计数技术测量培养基和头盖骨中的45CaCl2，并计算试验组和对照组半块头盖骨之间的吸收比率。该比率基于细胞介导的吸收，表明每种牙髓材料可溶性成分的毒性。根据文献，用我们的方法发现，在所测试的材料中，地塞米松毒性最大，牙胶毒性最小。这表明我们的模型可用于体外测试其他生物材料对骨细胞的毒性。
