Keipert P E, Chang T M
Biomater Med Devices Artif Organs. 1985;13(1-2):1-15. doi: 10.3109/10731198509118839.
In our previous work, pyridoxylated polyhemoglobin (PP-PolyHB) was shown to have a P50 = 16-18 torr, and a half-life (T 1/2) of 20 hrs in the circulation of rats given a 75% isovolemic exchange transfusion. For the present study, a rapid and lethal hemorrhagic shock model has been specifically designed to assess the ability of PP-PolyHB to function as an emergency resuscitation fluid. Using 48 fully conscious rats with special chronic arterial and venous cannulations, shock was induced by bleeding 67% of total blood volume in less than 40 min; producing 100% mortality in nonresuscitated controls. Resuscitation was carried out using one of the following infusion fluids equivalent in volume to the bled volume: Ringer's solution, albumin solution, stroma-free Hb (SFHb), pyridoxylated SFHb (PP-SFHb), PP-PolyHb, and whole blood. Long-term (greater than 8 day) survival rate of rats (n = 12) resuscitated with PP-PolyHb was 75% compared to 83% for autologous whole blood. Survival following resuscitation with the other fluids was substantially lower. These results indicate that PP-PolyHb could effectively resuscitate lethal hemorrhagic shock in conscious rats, and provide long-term survival afterwards, even in the absence of any additional fluid maintenance.
在我们之前的工作中，吡哆醛化多聚血红蛋白（PP - 多聚血红蛋白）在接受75%等容交换输血的大鼠循环中显示出P50 = 16 - 18托，半衰期（T1/2）为20小时。在本研究中，专门设计了一种快速致死性失血性休克模型，以评估PP - 多聚血红蛋白作为紧急复苏液的功能。使用48只具有特殊慢性动静脉插管的清醒大鼠，在不到40分钟内放血占总血容量的67%诱导休克；未复苏的对照组死亡率达100%。使用以下与放血量等体积的输注液之一进行复苏：林格氏液、白蛋白溶液、无基质血红蛋白（SFHb）、吡哆醛化无基质血红蛋白（PP - SFHb）、PP - 多聚血红蛋白和全血。用PP - 多聚血红蛋白复苏的大鼠（n = 12）的长期（大于8天）存活率为75%，而自体全血为83%。用其他液体复苏后的存活率则低得多。这些结果表明，PP - 多聚血红蛋白可有效复苏清醒大鼠的致死性失血性休克，并在之后提供长期存活，即使在没有任何额外液体维持的情况下也是如此。
