Ding Yi, Kowada Toshiyuki, Matsui Toshitaka, Sasaki Norihiko, Mizukami Shin
Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi 980-8577, Japan.
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi 980-8577, Japan.
Bioconjug Chem. 2025 Sep 17;36(9):1980-1986. doi: 10.1021/acs.bioconjchem.5c00251. Epub 2025 Aug 23.
Sulfatase 2 (Sulf-2), an extracellular sulfatase that modulates the sulfation pattern of heparan sulfate proteoglycans (HSPGs), serves as a critical biomarker for various pathological conditions. Monitoring Sulf-2 activity in living cell samples provides valuable insights for diagnostic applications and therapeutic evaluation of Sulf-2-related diseases. In this study, we developed a novel plasma-membrane-targeted fluorogenic probe, , to visualize the activity of Sulf-2 secreted by living cells. Upon incubation with Sulf-2-containing culture supernatant from pancreatic cancer cells, exhibited a significant increase in fluorescence at approximately 540 nm. Notably, allowed for time-lapse monitoring of endogenous Sulf-2 activity in living cancer cells overexpressing Sulf-2, demonstrating its potential as a valuable tool for Sulf-2-related cancer diagnostics and therapeutic research.
硫酸酯酶2（Sulf-2）是一种细胞外硫酸酯酶，可调节硫酸乙酰肝素蛋白聚糖（HSPGs）的硫酸化模式，是各种病理状况的关键生物标志物。监测活细胞样品中的Sulf-2活性可为Sulf-2相关疾病的诊断应用和治疗评估提供有价值的见解。在本研究中，我们开发了一种新型的靶向质膜的荧光探针，用于可视化活细胞分泌的Sulf-2的活性。与来自胰腺癌细胞的含Sulf-2的培养上清液孵育后，在约540nm处荧光显著增加。值得注意的是，该探针可对过表达Sulf-2的活癌细胞内源性Sulf-2活性进行延时监测，证明了其作为Sulf-2相关癌症诊断和治疗研究的有价值工具的潜力。
