Shao Yuxin, Suo Yanrui, Wang Zheng, Sun Huiling, Cui Yifang, Guo Fangfang, Lin Dongmei, Ma Tenghe, Xu Fuzhou
Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Hebei University of Engineering, Handan, Hebei Province 056038, PR China.
Poult Sci. 2025 Aug 19;104(11):105705. doi: 10.1016/j.psj.2025.105705.
The investigation aimed to reveal potential mechanisms of zinc methionine on the intestinal mucosal function of Salmonella-challenged broilers. Totally, 320 Ross 308 male chicks were divided into 4 treatments, with 8 repetition per group and 10 chicks each. A 2 × 2 factor in a randomized block design was employed to explore the impacts of zinc addition, Salmonella challenge, and their interactions. The experiment lasted for 21 days. Results indicated that Salmonella infection decreased (P < 0.05) average daily gain (ADG) and the ratio of villus height to crypt depth (V/C) in both jejunum and ileum. The infection also reduced (P < 0.05) MUC2 and KLF4 mRNA levels, while elevating (P < 0.05) feed to gain (F:G), caudal-related homeobox transcriptional regulator 2 (CDX2) mRNA level, and altering (P < 0.05) bacterial composition. In contrast, zinc addition increased (P < 0.05) ADG, average daily feed intake, jejunal villus height, V/C, and tight junction mRNA expressions, but decreased (P < 0.05) F:G and CDX2 mRNA level. Moreover, zinc enhanced (P < 0.05) jejunal goblet cell number and ileal mucus thickness, and restored (P < 0.05) bacterial abundance. Correlation analysis found that zinc-enriched bacterial genera, including g_Butyricicoccus, g_Oscillibacter and g_Monoglobus were significantly positively correlated with broilers' growth performance and intestinal morphology and tight junction Claudin-1 mRNA. CUT&Tag-seq analysis further demonstrated KLF4 binds to the exon region of MUC2 gene, but CDX2 did not bind to MUC2 promoter region. These suggest zinc mitigates Salmonella induced intestinal mucosal damage by promoting mucus secretion and tight junction mRNA expression, and microbial community reshaping, but not by improving KLF4-mediated MUC2 gene expression.
本研究旨在揭示蛋氨酸锌对受沙门氏菌攻击的肉鸡肠道黏膜功能的潜在作用机制。总共320只罗斯308雄性雏鸡被分为4组处理，每组8个重复，每组10只鸡。采用随机区组设计的2×2因子设计，以探究锌添加、沙门氏菌攻击及其相互作用的影响。实验持续21天。结果表明，沙门氏菌感染降低了（P<0.05）空肠和回肠的平均日增重（ADG）以及绒毛高度与隐窝深度之比（V/C）。感染还降低了（P<0.05）MUC2和KLF4 mRNA水平，同时提高了（P<0.05）料重比（F:G）、尾型相关同源盒转录调节因子2（CDX2）mRNA水平，并改变了（P<0.05）细菌组成。相反，添加锌增加了（P<0.05）ADG、平均日采食量、空肠绒毛高度、V/C和紧密连接mRNA表达，但降低了（P<0.05）F:G和CDX2 mRNA水平。此外，锌增加了（P<0.05）空肠杯状细胞数量和回肠黏液厚度，并恢复了（P<0.05）细菌丰度。相关性分析发现，富含锌的细菌属，包括丁酸球菌属、颤杆菌属和单球杆菌属，与肉鸡的生长性能、肠道形态和紧密连接Claudin-1 mRNA显著正相关。CUT&Tag-seq分析进一步表明，KLF4与MUC2基因的外显子区域结合，但CDX2不与MUC2启动子区域结合。这些结果表明，锌通过促进黏液分泌和紧密连接mRNA表达以及微生物群落重塑来减轻沙门氏菌诱导的肠道黏膜损伤，而不是通过改善KLF4介导的MUC2基因表达。
