Gangadhara Nikhil Y, B Manju, Kumar P Kiran, Krishnegowda Avinash, Krishna Honnur, Mahesh Lohith K S, Sadashivanna Ravishankar H, Ravikumar Raghavendra
Department of Chemistry, Maharaja Institute of Technology Mysore, Mandya, Karnataka 571438, India.
Department of Chemistry, Seshadripuram Institute of Technology, Mysore, Karnataka 571311, India.
J Pharm Biomed Anal. 2025 Dec 15;266:117119. doi: 10.1016/j.jpba.2025.117119. Epub 2025 Aug 16.
Oxygen deficient molybdenum oxide nanosheet (Od-MO Nshs) was synthesised by hydrothermal method and its peroxidase-like activity was established with 3,3',5,5'-Tetramethylbenzidine-(TMB) and o-phenylenediamine dihydrochloride-(OPD) as a co-substrate in presence of Hydrogen peroxide (HO) and catalytic parameters were compared with horseradish peroxidase-(HRP) enzyme. Optimization was carried out for physical and chemical parameters. Synthesised nanoparticles-(NPs) were characterized for size, shape, composition oxidation state etc. NPs crystalline size was calculated using Scherrer's equation and Williamson-Hall plot and was found to be 18.46 nm and 36.1 nm respectively. Based on the peroxidase-like activity of Od-MO Nshs nanozymes-(NZs), a simple colorimetric method was established for detecting HO in human serum samples. Compared to HRP, Od-MO NShs showed linear detection in the fixed time method at 48 times lower HO concentration with TMB and 16 times and 4 times lower HO concentration accordingly. Od-MO NShs showed 2.7- and 5.3-time lesser Michaelis-Menten constant (K) for HO compared to HRP with TMB and OPD, respectively indicating higher substrate affinity. The detection and quantification limits for TMB-Od-MO NShs were 0.5 and 1.5 µM, significantly lower than TMB-HRP (9.4 µM, 28.5 mM). For OPD-Od-MO NShs, the limits were 9.4 µM, and 28.5 m, compared to 1.1 µM and 3.4 µM for OPD-Od-MO NShs. in addition, these NPs exhibited photocatalytic performance, achieving effective degradation (>55 %) of selected organic dyes, highlighting their potential environmental remediation application.
采用水热法合成了缺氧氧化钼纳米片（Od-MO Nshs），并以3,3',5,5'-四甲基联苯胺（TMB）和盐酸邻苯二胺（OPD）作为共底物，在过氧化氢（HO）存在的情况下测定其类过氧化物酶活性，并将其催化参数与辣根过氧化物酶（HRP）进行比较。对物理和化学参数进行了优化。对合成的纳米颗粒（NPs）的尺寸、形状、组成氧化态等进行了表征。使用谢乐方程和威廉姆森-霍尔图计算了NPs的晶体尺寸，分别为18.46 nm和36.1 nm。基于Od-MO Nshs纳米酶（NZs）的类过氧化物酶活性，建立了一种简单的比色法用于检测人血清样本中的HO。与HRP相比，Od-MO NShs在固定时间法中对HO的线性检测浓度分别比TMB低48倍、比OPD低16倍和4倍。与HRP相比，Od-MO NShs对HO的米氏常数（K）分别低2.7倍和5.3倍，这表明其对底物的亲和力更高。TMB-Od-MO NShs的检测限和定量限分别为0.5和1.5 μM，显著低于TMB-HRP（9.4 μM，28.5 mM）。对于OPD-Od-MO NShs，其检测限和定量限分别为9.4 μM和28.5 mM，而OPD-HRP的检测限和定量限分别为1.1 μM和3.4 μM。此外，这些NPs表现出光催化性能，能够有效降解（>55%）选定的有机染料，突出了它们在环境修复方面的潜在应用价值。
