Yu Pengyan, Ma Chang, Gao Qi, Yan Dong, Liu Yuanyuan, Xu Jianping, Liu Yu, Zong Xianchun, Wang Shouxian, Fan Yangyang
Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Beijing 100097, China; College of Life Science and Technology, Mudanjiang Normal University, Mudanjiang 157011, China.
Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Beijing 100097, China; Inner Mongolia Agricultural University, Hohhot 010018, China.
Int J Biol Macromol. 2025 Sep;322(Pt 4):147032. doi: 10.1016/j.ijbiomac.2025.147032. Epub 2025 Aug 21.
The superoxide dismutase (SOD) enzymes are a group of key enzymes involved in oxidative stress defense and play a significant role in organismal growth and development. The SOD gene family has been identified in many plants, animals, and some fungi but not in the economically important edible mushroom Lentinula edodes. In this study, members of the SOD gene family of L. edodes were identified and analyzed using bioinformatic methods. Three proteins containing signature SOD domains were identified in each of the two monokaryotic strains SP3 and SP30 of L. edodes, and all six proteins belonged to the major Fe/Mn-SOD type. Phylogenetic analysis revealed that these LeSOD genes were classified into two subtypes, with members of each type sharing the same conserved motifs. Transcriptome data of mycelia grown under different conditions showed that LeSOD1 was significantly up-regulated under heat stress, while LeSOD2 was significantly up-regulated under hydrogen peroxide stress. Together, the genomic and transcriptomic analyses suggest that LeSOD1 and LeSOD2 are key genes for L. edodes to respond to abiotic stresses. This study provides a foundation for understanding both the roles of the SOD gene family and the molecular mechanism by which L. edodes responds to heat and oxidative stresses.
超氧化物歧化酶（SOD）是参与氧化应激防御的一组关键酶，在生物体的生长发育中发挥着重要作用。SOD基因家族已在许多植物、动物和一些真菌中被鉴定出来，但在经济上重要的食用菌香菇中尚未被发现。在本研究中，利用生物信息学方法对香菇SOD基因家族成员进行了鉴定和分析。在香菇的两个单核菌株SP3和SP30中，每个菌株都鉴定出了三种含有标志性SOD结构域的蛋白质，这六种蛋白质均属于主要的铁/锰-SOD类型。系统发育分析表明，这些香菇SOD基因被分为两个亚型，每个亚型的成员具有相同的保守基序。不同条件下生长的菌丝体的转录组数据表明，LeSOD1在热胁迫下显著上调，而LeSOD2在过氧化氢胁迫下显著上调。基因组和转录组分析共同表明，LeSOD1和LeSOD2是香菇应对非生物胁迫的关键基因。本研究为理解SOD基因家族的作用以及香菇应对热胁迫和氧化胁迫的分子机制奠定了基础。
